| 货号 | 2454S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-P |
| 目标/特异性 | Beta-Amyloid Antibody detects several isoforms of beta-amyloid peptide (Abeta), such as Abeta-40, Abeta-42 etc, regardless of phosphorylation state. |
| 使用方法 | WB(1:1000) IHC-P (1:200) IF-P (1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Amyloid β (A4) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms (1). The amino acid sequence of APP contains the amyloid domain (A-β), which can be released by a two-step proteolytic cleavage (1). The extracellular deposition and accumulation of the released A-β fragments form the main components of amyloid plaques in Alzheimer disease (1). APP can be phosphorylated at several sites, which may affect the proteolytic processing and secretion of this protein (2-5). The phosphorylation at Thr668 (at a position corresponding to the APP695 isoform) by cyclin-dependent kinase is cell cycle dependent and peaks during G2/M-phase (4). The APP Thr668 phosphorylated form exists in adult rat brain and correlates with cultured neuronal differentiation (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 5 |
| 参考文献 | 1 . Selkoe, D.J. (1996) J Biol Chem 271, 18295-8. 2 . Caporaso, G.L. et al. (1992) Proc Natl Acad Sci USA 89, 3055-9. 3 . Hung, A.Y. and Selkoe, D.J. (1994) EMBO J 13, 534-42. 4 . Suzuki, T. et al. (1994) EMBO J 13, 1114-22. 5 . Ando, K. et al. (1999) J Neurosci 19, 4421-7. 6 . Iijima, K. et al. (2000) J Neurochem 75, 1085-91. |
Western blot analysis of human A-beta-40 and A-beta-42 peptides (1-5 ng), using beta-Amyloid Antibody. Western blot分析人A-beta-40和A-beta-42肽段（1-5ng），使用的抗体是beta-Amyloid Antibody 兔多抗。 | |
Confocal immunofluorescent analysis of human Alzheimers brain, using beta-Amyloid Antibody (red) and Tau (Tau46) Mouse mAb #4019 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析人阿尔茨海默病大脑，使用beta-Amyloid Antibody 兔多抗（红色）和Tau (Tau46) Mouse mAb 鼠单抗 #4019（绿色）进行标记。蓝色伪彩=DRAQ5®#4084(荧光DNA染料)。 | |
Immunohistochemical analysis of paraffin-embedded Alzheimers brain, using beta-Amyloid Antibody. 免疫组化分析石蜡包埋的阿尔茨海默病大脑，使用的抗体是beta-Amyloid Antibody 兔多抗。 |
