| 货号 | 3134S |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | α-Actinin Antibody detects endogenous levels of total α-actinin-1 protein. Based on sequence homology, the antibody may cross-react with α-actinin isoforms 2 and 4. |
| 使用方法 | WB(1:1000) IF-IC (1:25) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | α-actinin belongs to the spectrin family of cytoskeletal proteins. It was first recognized as an actin cross-linking protein, forming an antiparallel homodimer with an actin binding head at the amino terminus of each monomer. More recently, α-actinin has been shown to interact with a large number of proteins involved in signaling to the cytoskeleton including those involved in cellular adhesion, migration and immune cell targeting. (1). The interaction of α-actinin with ICAM5 (intercellular adhesion molecule-5) helps to promote neurite outgrowth (2). In osteoblasts, interaction of α-actinin with integrins stabilizes focal adhesions and may protect cells from apoptosis (3). Isoforms 1 and 4 of α-actinin, which are non-muscle isoforms, are present in stress fibers, sites of adhesion and intercellular contacts, filopodia and lamellipodia. The muscle isoforms 2 and 3 localize to the Z-discs of striated muscle and to dense bodies and plaques in smooth muscle (1).α-actinin蛋白属于细胞骨架蛋白的血影蛋白家族。其首先被确认作为一个actin交联蛋白，与actin蛋白每个单体的氨基末端的连接头形成一个反向平行的二聚体。最近，α-actinin蛋白已经研究显示与大量蛋白相互作用，其涉及到细胞骨架信号包括细胞粘附、迁移及免疫细胞定位(1)。α-actinin蛋白与ICAM5 (intercellular adhesion molecule-5)的相互作用有助于促进轴突生长(2)。在成骨细胞中，α-actinin与integrins的相互作用稳定粘着斑，并且有助于阻止细胞凋亡(3)。 α-actinin蛋白的亚型1和4是非肌肉特异性亚型，它们存在于应力纤维，粘连的部位和细胞间的接触，丝状伪足和片状伪足。肌肉特异性亚型2和3定位于横纹肌Z-discs、致密体和平滑肌斑块(1)。 |
| 存放说明 | -20C |
| 计算分子量 | 100 |
| 参考文献 | 1 . Otey, C.A. and Carpen, O. (2004) Cell Motil Cytoskeleton 58, 104-11. 2 . Nyman-Huttunen, H. et al. (2006) J Cell Sci 119, 3057-66. 3 . Triplett, J.W. and Pavalko, F.M. (2006) Am J Physiol Cell Physiol 291, C909-21. |
Western blot analysis of extracts from NIH/3T3, C6, CHO and COS cells using α-Actinin Antibody.使用α-Actinin Antibody，免疫印迹（Western Blot）分析NIH/3T3、C6、CHO和COS细胞系β-Actin蛋白水平。 | |
Confocal immunofluorescent analysis of NIH/3T3 cells using alpha-Actinin Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).使用alpha-Actinin Antibody（绿色）标记，共聚焦免疫荧光（immunofluorescent）分析NIH/3T3细胞。Alexa Fluor® 555 phalloidin标记Actin filaments（红色）。蓝色= DRAQ5™（DNA荧光染料）。 |
