| 货号 | 4202S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC |
| 目标/特异性 | YB1 (D299) Antibody detects endogenous levels of total YB1 protein. |
| 使用方法 | WB(1:1000) IHC-P (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The Y-box binding protein 1 (YB1) belongs to a family of evolutionarily conserved, multifunctional Y-box proteins that bind single-stranded DNA and RNA and function as regulators of transcription, RNA metabolism, and protein synthesis (1). YB1 binds to Y-box sequences (TAACC) found in multiple gene promoters and can positively or negatively regulate transcription. YB1 activates genes associated with proliferation and cancer, such as cyclin A, cyclin B1, matrix metalloproteinase-2 (MMP-2), and the multi-drug resistance 1 (MDR1) gene (2-4). YB1 represses genes associated with cell death, including the Fas cell death-associated receptor and the p53 tumor suppressor gene (5-7). It also interacts with the RNA-splicing factor SRp30c and stabilizes interleukin 2 mRNA upon induction of T lymphocytes by interleukin 2 (8,9). The majority of YB1 protein localizes to the cytoplasm, with a minor pool found in the nucleus; however, nuclear localization appears to be critical for its role in promoting proliferation. Nuclear translocation is cell cycle regulated, with YB1 protein accumulating in the nucleus during G1/S phase (2). In addition, nuclear translocation is induced in response to extracellular stimuli such as hyperthermia and UV irradiation, or treatment of cells with thrombin, interferons, or insulin-like growth factor (IGF-1) (2,10). Treatment of the MCF7 breast cancer cell line with IGF-1 results in Akt-mediated phosphorylation of YB1 on Ser102, which is required for nuclear translocation of YB1 and its ability to promote anchorage-independent growth (10). YB1 is overexpressed in many malignant tissues, including breast cancer, non-small cell lung carcinoma, ovarian adenocarcinomas, human osteosarcomas, colorectal carcinomas, and malignant melanomas. Nuclear YB1 expression correlates with high levels of proliferation, drug resistance, and poor tumor prognosis (2,7,10). |
| 存放说明 | -20C |
| 计算分子量 | 49 |
| 参考文献 | 1 . Matsumoto, K. and Wolffe, A.P. (1998) Trends Cell Biol. 8, 318-23. 2 . Jurchott, K. et al. (2003) J. Biol. Chem. 278, 27988-96. 3 . Mertens, P.R. et al. (1997) J. Biol. Chem. 272, 22905-12. 4 . Uchiumi, T. et al. (1993) Cell Growth Differ. 4, 147-57. 5 . Lasham, A. et al. (2000) Gene 252, 1-13. 6 . Lasham, A. et al. (2003) J. Biol. Chem. 278, 35516-23. 7 . Homer, C. et al. (2005) Oncogene 24, 8314-25. 8 . Raffetseder, U. et al. (2003) J. Biol. Chem. 278, 18241-8. 9 . Chen, C.Y. et al. (2000) Genes Dev. 14, 1236-48. 10 . Sutherland, B.W. et al. (2005) Oncogene 24, 4281-92. |
Western blot analysis of extracts from various cell lines using YB1 (D299) Antibody. 应用YB1 (D299)抗体Western blot 分析各种细胞的提取物，所用抗体为 YB1 (D299) Antibody。 | |
Confocal immunofluorescent analysis of HeLa cells using YB1 (D299) Antibody (green). Actin filaments were labeled using DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 应用YB1 (D299)（绿色）免疫荧光共聚焦分析HeLa细胞。肌动蛋白纤维是用DY-554 phalloidin （红色）标记。蓝色伪彩为DRAQ5® #4084 （DNA荧光染料） | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using YB1 (D299) Antibody in the presence of control peptide (left) or antigen-specific peptide (right). 免疫组化分析石蜡包埋的人乳腺癌细胞，分别在对照肽段（左）和抗原特异性肽段（右）下使用YB1 (D299)抗体标记。 |
