| 货号 | 4912S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P |
| 目标/特异性 | YAP Antibody detects endogenous levels of total YAP protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | YAP (Yes-associated protein, YAP65) was identified based on its ability to associate with the SH3 domain of Yes. It also binds to other SH3 domain-containing proteins such as Nck, Crk, Src, and Abl (1). In addition to the SH3 binding motif, YAP contains a PDZ interaction motif, a coiled-coil domain, and WW domains (2-4). While initial studies of YAP all pointed towards a role in anchoring and targeting to specific subcellular compartments, subsequent studies showed that YAP is a transcriptional co-activator by virtue of its WW domain interacting with the PY motif (PPxY) of the transcription factor PEBP2 and other transcription factors (5,6). Upon phosphorylation at Ser127, YAP interacts with 14-3-3 in an Akt-dependent manner and suppresses p73-mediated apoptosis (6). |
| 存放说明 | -20C |
| 计算分子量 | 65 to 75 |
| 参考文献 | 1 . Sudol, M. (1994) Oncogene 9, 2145-52. 2 . Mohler, P.J. et al. (1999) J Cell Biol 147, 879-90. 3 . Espanel, X. and Sudol, M. (2001) J Biol Chem 276, 14514-23. 4 . Sudol, M. et al. (1995) FEBS Lett 369, 67-71. 5 . Yagi, R. et al. (1999) EMBO J 18, 2551-62. 6 . Basu, S. et al. (2003) Mol Cell 11, 11-23. 7 . Zhao, B. et al. (2010) Genes Dev 24, 862-74. 8 . Zhao, B. et al. (2007) Genes Dev 21, 2747-61. 9 . Yu, F.X. et al. (2012) Cell 150, 780-91. 10 . Zhao, B. et al. (2010) Genes Dev 24, 72-85. |
Western blot analysis of extracts from NIH/3T3, C6 and MDA-MB-231 cells, using YAP Antibody.使用YAP抗体对 NIH/3T3, C6 和MDA-MB-231细胞提取物进行Western blot分析。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic and nuclear localization, using YAP Antibody.使用YAP抗体对石蜡包埋的人乳腺癌组织进行免疫组化分析，显示细胞质和细胞核定位。 | |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, showing cytoplasmic and nuclear localization, using YAP Antibody.使用YAP抗体对石蜡包埋的人前列腺癌组织进行免疫组化分析，显示细胞质和细胞核定位。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using YAP Antibody in the presence of control peptide (left) or antigen specific peptide (right).在对照多肽（左）或抗原特异性多肽（右）存在的条件下用YAP抗体对石蜡包埋的人结肠癌组织进行免疫组化分析。 |
