| 货号 | 12124S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | WIPI1 Antibody recognizes endogenous levels of total WIPI1 protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). It is generally activated by conditions of nutrient deprivation but is also associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and is directed by a number of autophagy-related (Atg) genes. Vacuolar trafficking and autophagy are controlled by the class III type phosphoinositide 3-kinase (PI3K) Vps34, which generates phosphoinositide-3-phosphate (PtdIns3P) (4,5). Atg18 and Atg21 are two related WD-repeat proteins that bind PtdIns3P via a conserved Phe-Arg-Arg-Gly motif (6,7). It has been shown that Atg18 binds to Atg2 and that this complex is directed to vacuolar membranes by its interaction with PtdIns3P (8). Human orthologues of Atg18 and Atg21 were identified as members of the WD-repeat protein Interacting with PhosphoInositides (WIPI) family (9-11). WIPI1 (also called WIPI49) and WIPI2 have been shown to translocate from several vacuolar compartments to LC3-positive autophagosomes during autophagy; this translocation may be used as an autophagy marker (12). |
| 存放说明 | -20C |
| 计算分子量 | 48 |
| 参考文献 | 1 . Reggiori, F. and Klionsky, D.J. (2002) Eukaryot. Cell 1, 11-21. 2 . Codogno, P. and Meijer, A.J. (2005) Cell Death Differ. 12 Suppl 2, 1509-18. 3 . Levine, B. and Yuan, J. (2005) J. Clin. Invest. 115, 2679-88. 4 . Corvera, S. (2001) Traffic 2, 859-66. 5 . Yan, Y. and Backer, J.M. (2007) Biochem Soc Trans 35, 239-41. 6 . Krick, R. et al. (2006) FEBS Lett 580, 4632-8. 7 . Strømhaug, P.E. et al. (2004) Mol Biol Cell 15, 3553-66. 8 . Obara, K. et al. (2008) J Biol Chem 283, 23972-80. 9 . Jeffries, T.R. et al. (2004) Mol Biol Cell 15, 2652-63. 10 . Proikas-Cezanne, T. et al. (2007) FEBS Lett 581, 3396-404. 11 . Polson, H.E. et al. (2010) Autophagy 6, 506-22. 12 . Proikas-Cezanne, T. et al. (2007) FEBS Lett 581, 3396-404. |
Western blot analysis of extracts from various cell lines using WIPI1 Antibody. Westerm blot 分析多种细胞系的细胞提取物，使用抗体是WIPI1 Antibody。 | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing human WIPI1 (hWIPI1; +), using WIPI1 Antibody. Western blot 分析293T细胞提取物，对照转染(-)或转染表达人源WIPI1的质粒(hWIPI1; +)，使用抗体为WIPI1 Antibody。 |
