| 货号 | 4860S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | WASP Antibody detects endogenous levels of total WASP protein. The antibody does not cross-react with N-WASP. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Wiskott-Aldrich syndrome proteins (WASPs) mediate actin dynamics by activating the Arp2/3 actin nucleation complex in response to activated Rho family GTPases. In mammals, five WASP family members have been described. Hematopoietic WASP and ubiquitously expressed N-WASP are autoinhibited in unstimulated cells. Upon stimulation they are activated by cdc42, which relieves the autoinhibition in conjunction with phosphatidyl inositol 4,5-bisphosphate. Three WAVE (Wasf, SCAR) family proteins are similar in sequence to WASP and N-WASP but lack the WASP/N-WASP autoinhibition domains and are indirectly activated by Rac (reviewed in 1). Both WASP and WAVE functions appear to be essential, as knockout of either N-WASP or Scar-2 in mice results in cardiac and neuronal defects and embryonic lethality (2,3). Loss of WASP results in immune system defects and fewer immune cells (4). WAVE-2 (WASF2) is widely distributed, while WAVE-1 and WAVE-3 are strongly expressed in brain (5). WAVE-3 may act as a tumor suppressor in neuroblastoma, a childhood disease of the sympathetic nervous system (6). Increased expression of WAVE-3 is seen in breast cancer, and studies in breast adenocarcinoma cells indicate that WAVE-3 regulates breast cancer progression, invasion and metastasis through the p38 mitogen-activated protein kinase (MAPK) pathway (7,8). |
| 存放说明 | -20C |
| 计算分子量 | 60 human, 62 mouse |
| 参考文献 | 1 . Millard, T.H. et al. (2004) Biochem J 380, 1-17. 2 . Yan, C. et al. (2003) EMBO J. 22, 3602-3612. 3 . Snapper, S.B. et al. (2001) Nat. Cell Biol. 3, 897-904. 4 . Zhang, J. et al. (1999) J. Exp. Med. 190, 1329-4132. 5 . Suetsugu, S. et al. (1999) Biochem Biophys Res Commun 260, 296-302. 6 . Sossey-Alaoui, K. et al. (2002) Oncogene 21, 5967-5974. 7 . Sossey-Alaoui, K. et al. (2005) Exp. Cell Res. 308, 135-145. 8 . Sossey-Alaoui, K. et al. (2007) Am J Pathol 170, 2112-21. |
Western blot analysis of extracts from various cell lines, using WASP Antibody. 使用WASP Antibody，免疫印迹（Western Blot）分析不同细胞中WASP蛋白水平。 | |
Immunoprecipitation of WASP from Ramos cells, followed by western blot analysis, using WASP Antibody. 使用WASP Antibody，免疫陈淀分析Ramos细胞中WASP蛋白，随后进行免疫印迹实验分析。 |
