| 货号 | 3932S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | Vimentin (R28) Antibody detects endogenous levels of total vimentin protein. |
| 使用方法 | WB(1:1000) IF-IC (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments, and microtubules. Major types of intermediate filaments are distinguished by their cell specific expression: cytokeratins (epithelial cells), glial fibrillary acidic protein (GFAP) (glial cells), desmin (skeletal, visceral, and certain vascular smooth muscle cells), vimentin (mesenchyme origin), and neurofilaments (neurons). GFAP and vimentin form intermediate filaments in astroglial cells and modulate their motility and shape (1). In particular, vimentin filaments are present at early developmental stages, while GFAP filaments are characteristic of differentiated and mature brain astrocytes. Thus, GFAP is commonly used as a marker for intracranial and intraspinal tumors arising from astrocytes (2). Vimentin is present in sarcomas, but not carcinomas, and its expression is examined in conjunction with that of other markers to distinguish between the two (3). Vimentins dynamic structural changes and spatial re-organization in response to extracellular stimuli help to coordinate various signaling pathways (4). Phosphorylation of vimentin at Ser56 in smooth muscle cells regulates the structural arrangement of vimentin filaments in response to serotonin (5,6). Remodeling of vimentin and other intermediate filaments is important during lymphocyte adhesion and migration through the endothelium (7). |
| 存放说明 | -20C |
| 计算分子量 | 57, 50 |
| 参考文献 | 1 . Eng, L.F. et al. (2000) Neurochem Res 25, 1439-51. 2 . Goebel, H.H. et al. (1987) Acta Histochem Suppl 34, 81-93. 3 . Leader, M. et al. (1987) Histopathology 11, 63-72. 4 . Helfand, B.T. et al. (2004) J Cell Sci 117, 133-41. 5 . Tang, D.D. et al. (2005) Biochem J 388, 773-83. 6 . Fomina, I.G. et al. (1990) Klin Med (Mosk) 68, 125-7. 7 . Nieminen, M. et al. (2006) Nat Cell Biol 8, 156-62. 8 . Yamaguchi, T. et al. (2005) J Cell Biol 171, 431-6. 9 . Oguri, T. et al. (2006) Genes Cells 11, 531-40. 10 . Zhu, Q.S. et al. (2011) Oncogene 30, 457-70. 11 . Xue, G. and Hemmings, B.A. (2013) J Natl Cancer Inst 105, 393-404. |
Confocal immunofluorescent analysis of HeLa cells using Vimentin (R28) Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用Vimentin (R28) Antibody (绿色)标记，共聚焦免疫荧光分析HeLa细胞。DY-554 phalloidin标记微丝蛋白(红色)。蓝色伪彩= DRAQ5® #4084 (DNA荧光染料)。 | |
Western blot analysis of extracts from various cell lines using Vimentin (R28) Antibody. 使用Vimentin (R28) Antibody，免疫印迹（Western Blot）分析不同细胞中Vimentin (R28)蛋白水平。 |
