| 货号 | 2369S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | Villin-1 (R814) Antibody recognizes endogenous levels of total villin-1 protein. |
| 使用方法 | WB(1:1000) IF-IC (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Villin is a member of the gelsolin family of calcium-regulated actin-binding proteins. Unlike the ubiquitously expressed gelsolin, villin expression is restricted to simple epithelia of the gastrointestinal and urogenital tracts. It is localized to the apical cytoplasm and brush borders of these cells. Villin functions in the regulation of actin dynamics in the apical epithelium, capping, nucleating and/or severing actin filaments in a calcium-dependent manner and regulating cell shape in response to external stimuli (1,2). Phosphorylation of villin at Tyr60, 81 and 256 may be involved in the regulation of cell migration (3). Expression of villin is increased in colorectal cancers (4), and villin1 function appears to be involved in progressive cholestasis and hepatic failure (5). |
| 存放说明 | -20C |
| 计算分子量 | 95 |
| 参考文献 | 1 . Athman, R. et al. (2002) Am. J. Physiol. Gastrointest. Liver Physiol. 283, G496-502. 2 . Kumar, N. et al. (2004) J. Biol. Chem. 279, 45036-45046. 3 . Tomar, A. et al. (2004) Mol. Biol. Cell 15, 4807-4817. 4 . Suh, N. et al. (2005) Mod. Pathol. 18, 1217-1222. 5 . Phillips, M.J. et al. (2003) Lancet 362, 1112-1119. |
Confocal immunofluorescent analysis of HT29 cells using Villin-1 (R814) Antibody (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). 使用Villin-1 (R814) Antibody (绿色)标记，共聚焦免疫荧光分析HT29细胞。蓝色伪彩= DRAQ5™ (DNA荧光染料)。 | |
Western blot analysis of extracts from HT29 cells and mouse kidney using Villin-1 (R814) Antibody. 使用Villin-1 (R814) Antibody，免疫印迹(Western blot)分析HT29细胞和小鼠肾脏组织中Villin-1蛋白水平。 |
