| 货号 | 2158S |
| 反应种属 | Human/D.melanogaster/Bovine |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | VE-Cadherin Antibody detects endogenous levels of total VE-cadherin protein. The antibody does not cross-react with other cadherin family members. |
| 使用方法 | WB(1:1000) IF-IC (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B- and E-cadherins as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking (1-4). E-cadherin is considered an active suppressor of invasion and growth of many epithelial cancers (1-3). Recent studies indicate that cancer cells have up-regulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch". N-cadherin cooperates with the FGF receptor, leading to over-expression of MMP-9 and cellular invasion (3). In endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8). |
| 存放说明 | -20C |
| 计算分子量 | 130-140 |
| 参考文献 | 1 . Wheelock, M.J. and Johnson, K.R. (2003) Annu Rev Cell Dev Biol 19, 207-35. 2 . Christofori, G. (2003) EMBO J 22, 2318-23. 3 . Hazan, R.B. et al. (2004) Ann N Y Acad Sci 1014, 155-63. 4 . Bryant, D.M. and Stow, J.L. (2004) Trends Cell Biol 14, 427-34. 5 . Rabascio, C. et al. (2004) Cancer Res 64, 4373-7. 6 . Yamaoka-Tojo, M. et al. (2006) Arterioscler Thromb Vasc Biol 26, 1991-7. 7 . Patel, I.S. et al. (2003) Int J Cancer 106, 172-7. 8 . Sanders, D.S. et al. (2000) J Pathol 190, 526-30. |
Western blot analysis of extracts from various cell types, using VE-Cadherin Antibody.用VE-Cadherin 抗体对多种细胞提取物进行western blot分析。 | |
Confocal immunofluorescent analysis of HUVE cells, using VE-Cadherin Antibody (green) and MEK1/2 (L38C12) Mouse mAb #4694 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用VE-Cadherin 抗体（绿色）和MEK1/2（L38C12）小鼠单抗#4694（红色）对HUVE细胞进行激光共聚焦免疫荧光分析。蓝色假色=DRAQ5® #4084 （DNA荧光分析）。 |
