| 货号 | 14694S |
| 反应种属 | Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | VCAM-1 Antibody (Rodent Specific) recognizes endogenous levels of total VCAM-1 protein. This antibody recognizes both long and short isoforms of VCAM-1 protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | VCAM-1 (vascular cell adhesion molecule-1) is a transmembrane glycoprotein containing multiple amino-terminal extracellular Ig-like domains, a transmembrane domain, and a short carboxy-terminal cytoplasmic domain (1). Alternative splicing generates two isoforms of VCAM-1 (2). The role of VCAM-1 during infection and inflammatory diseases is well characterized. Expression of VCAM-1 is induced in endothelial cells by inflammatory cytokines including TNF-α and IL-1β (1). VCAM-1 on endothelial cells interacts with the integrin VLA-4 (α4β1) on leukocytes to mediate migration of circulating leukocytes from the blood across the endothelium and into tissues (3). |
| 存放说明 | -20C |
| 计算分子量 | 35-45, 85-100 |
| 参考文献 | 1 . Osborn, L. et al. (1989) Cell 59, 1203-11. 2 . Hession, C. et al. (1991) J Biol Chem 266, 6682-5. 3 . Elices, M.J. et al. (1990) Cell 60, 577-84. |
Western blot analysis of extracts from MS-1 cells, untreated (-) or treated with Mouse Tumor Necrosis Factor-α (mTNF-α) #5178 (10 ng/ml, 16 hr; +), using VCAM-1 Antibody (Rodent Specific) (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Western blot analysis of extracts from 3T3, C2C12, and YB2/0 cells using VCAM-1 Antibody (Rodent Specific). | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length mouse VCAM-1 protein (mVCAM-1-Myc/DDK; +), using VCAM-1 Antibody (Rodent Specific). |
