| 货号 | 3112S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | VASP Antibody detects endogenous levels of total VASP protein. The antibody does not cross-react with VASP homologues, such as EVL and Mena. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Vasodilator-stimulated phosphoprotein (VASP) was originally characterized as a substrate of both cGMP- and cAMP-dependent kinases (PKG and PKA, or cGPK and cAPK, respectively) (1). It is now believed that VASP belongs to the Ena/VASP family of adaptor proteins linking the cytoskeletal system to the signal transduction pathways and that it functions in cytoskeletal organization, fibroblast migration, platelet activation and axon guidance (2,3). Three phosphorylation sites, Ser157, Ser239, and Thr278, have been identified. Ser239 is the major PKG phosphorylation site while Ser157 is the major PKA phosphorylation site (4). Evidence suggests that VASP phosphorylation reduces its association with actin and has a negative effect on actin polymerization (5). Phosphorylation at Ser239 of VASP is a useful marker for monitoring PKG activation and signaling (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 46, 50 |
| 参考文献 | 1 . Butt, E. et al. (1994) J Biol Chem 269, 14509-17. 2 . Ball, L.J. et al. (2000) EMBO J 19, 4903-14. 3 . Machesky, L.M. (2000) Cell 101, 685-8. 4 . Smolenski, A. et al. (1998) J Biol Chem 273, 20029-35. 5 . Harbeck, B. et al. (2000) J Biol Chem 275, 30817-25. 6 . Oelze, M. et al. (2000) Circ Res 87, 999-1005. 7 . Lawrence, D.W. and Pryzwansky, K.B. (2001) J Immunol 166, 5550-6. |
Western blot analysis of extracts from untreated THP-1 cells, untreated and forskolin treated C6 cells, and untreated C2C12 and COS cells, using the VASP Antibody. 使用VASP Antibody，免疫印迹(Western blot)分析未处理的THP-1细胞、未处理和forskolin处理的C6细胞以及未处理的C2C12和COS细胞。 |
