| 货号 | 13060S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | VAMP8 Antibody recognizes endogenous levels of total VAMP8 protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Proteins in the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex are integral membrane proteins involved in vesicle transport and membrane fusion by pairing of vesicular SNAREs (v-SNAREs) with cognate target SNAREs (t-SNAREs) (reviewed in 1,2). Vesicle associated membrane protein 8 (VAMP8), also known as endobrevin, is a v-SNARE originally found preferentially localized to early endosomes (3). VAMP8 knockout mice did not show abnormal endosomal vesicular trafficking, perhaps having a redundant role with other VAMP family members (4). Instead, research studies have shown that VAMP8 is widely expressed in exocrine tissues and has a critical role in the exocytosis pathways of a variety of cells (4-9). In addition, lysosome localized VAMP8 has been shown to play a role in autophagosome/lysosome fusion during antimicrobial (xenophagy) and canonical starvation induced autophagy (10). |
| 存放说明 | -20C |
| 计算分子量 | 15 |
| 参考文献 | 1 . Jena, B.P. (2011) Adv Exp Med Biol 713, 13-32. 2 . Kasai, H. et al. (2012) Physiol Rev 92, 1915-64. 3 . Wong, S.H. et al. (1998) Mol Biol Cell 9, 1549-63. 4 . Wang, C.C. et al. (2007) Mol Biol Cell 18, 1056-63. 5 . Furuta, N. et al. (2010) Mol Biol Cell 21, 1001-10. 6 . Nagamatsu, S. et al. (2001) J Cell Sci 114, 219-227. 7 . Okayama, M. et al. (2009) Cell Struct Funct 34, 115-25. 8 . Jones, L.C. et al. (2012) J Physiol 590, 545-62. 9 . Wang, C.C. et al. (2004) Dev Cell 7, 359-71. 10 . Furuta, N. et al. (2010) Mol Biol Cell 21, 1001-10. |
Western blot analysis of extracts from various cell lines using VAMP8 Antibody. | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length mouse VAMP8 (mVAMP8; +), using VAMP8 Antibody. |
