| 货号 | 13640S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | VAMP3 recognizes endogenous levels of total VAMP3 protein. This antibody does not cross-react with VAMP1 or VAMP2 proteins. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Proteins in the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex are integral membrane proteins involved in vesicle transport and membrane fusion by pairing of vesicular SNAREs (v-SNAREs) with cognate target SNAREs (t-SNAREs) (reviewed in 1,2). Vesicle associated membrane protein 3 (VAMP3), also known as cellubrevin, has a broad tissue distribution and localizes to endosomal compartments (3). VAMP3 interacts with the t-SNAREs syntaxin1, syntaxin4, SNAP23, and SNAP25 (4,5). Research studies indicate that VAMP3 is involved in transferrin receptor recycling to the plasma membrane (6) and in T-cell receptor recycling to immunological synapses (7). Inhibition of VAMP3 with tetanus toxin impairs membrane trafficking during cell migration (8). |
| 存放说明 | -20C |
| 计算分子量 | 13 |
| 参考文献 | 1 . Jena, B.P. (2011) Adv Exp Med Biol 713, 13-32. 2 . Kasai, H. et al. (2012) Physiol Rev 92, 1915-64. 3 . McMahon, H.T. et al. (1993) Nature 364, 346-9. 4 . Chilcote, T.J. et al. (1995) J Cell Biol 129, 219-31. 5 . Schraw, T.D. et al. (2003) Biochem J 374, 207-17. 6 . Galli, T. et al. (1994) J Cell Biol 125, 1015-24. 7 . Das, V. et al. (2004) Immunity 20, 577-88. 8 . Tayeb, M.A. et al. (2005) Exp Cell Res 305, 63-73. |
Western blot analysis of extracts from various cell lines using VAMP3 Antibody. | |
Immunoprecipitation of VAMP3 from SK-MEL-28 cell extracts using Normal Rabbit IgG #2729 (lane 2) or VAMP3 Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using VAMP3 Antibody. |
