| 货号 | 13151S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | VAMP1 Antibody recognizes endogenous levels of total VAMP1 protein. This antibody does not cross-react with VAMP2. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Vesicle-associated membrane protein 1 (VAMP1), also called synaptobrevin 1, is part of the R-soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex (1). The SNARE complex is involved in calcium regulated vesicular transport and membrane fusion (2). While related protein VAMP2 exhibits a wider distribution and is more abundant in the brain, VAMP1 is the main isoform in specific brain regions including the subthalamus nucleus zona incerta (1), the ostral periolivary region, and the retina (3). In addition, VAMP1 is involved in neurotransmitter release at the neuromuscular junction (4) and in the release of bioactive peptides from cardiac myocytes (5). |
| 存放说明 | -20C |
| 计算分子量 | 16 |
| 参考文献 | 1 . Raptis, A. et al. (2005) J Chem Neuroanat 30, 201-11. 2 . Sudhof, T.C. (2004) Annu Rev Neurosci 27, 509-47. 3 . Nystuen, A.M. et al. (2007) Neurogenetics 8, 1-10. 4 . Liu, Y. et al. (2011) J Physiol 589, 1603-18. 5 . Ferlito, M. et al. (2010) J Mol Cell Cardiol 49, 791-800. |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full length human VAMP1 (hVAMP1-Myc; +) or VAMP2 (hVAMP2-Myc; +), using VAMP1 Antibody (upper) and Myc-Tag (71D10) Rabbit mAb #2278 (lower). Western blot分析模拟转染(-)或转染了表达Myc标记全长人VAMP1 (hVAMP1-Myc; +) 或 VAMP2 (hVAMP2-Myc; +)质粒的293T细胞提取物，使用的抗体是VAMP1 Antibody 兔多抗 (上) 和Myc-Tag (71D10) Rabbit mAb 兔单抗#2278 (下)。 | |
Immunoprecipitation of VAMP1 from mouse brain using Normal Rabbit IgG #2729 (lane 2) or VAMP1 Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using VAMP1 Antibody. |
