| 货号 | 5320S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | UVRAG Antibody detects endogenous levels of total UVRAG protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). It is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. These proteins are involved in the formation of cytoplasmic vacuoles called autophagasomes that are delivered to lysosomes for degradation. The class III type phosphoinositide 3-kinase (PI3KC3)/Vps34 regulates vacuolar trafficking as well as autophagy (4,5). Multiple proteins have been shown to be associated with Vsp34, including: p105/Vsp15, Beclin-1, UVRAG, Atg14, and Rubicon, which can determine Vsp34 function (6-11). UVRAG (UV radiation resistance-associated gene) is associated with the Beclin-1/PI3KC3 complex and promotes PI3KC3 enzymatic activity and autophagy, while suppressing proliferation (11). Beclin-1 binding to UVRAG promotes both autophagosome maturation and endocytic trafficking (12). UVRAG is also a potential tumor suppressor protein with frameshift mutations observed in colon and gastric carcinomas (13,14). |
| 存放说明 | -20C |
| 计算分子量 | 90 |
| 参考文献 | 1 . Levine, B. and Yuan, J. (2005) J. Clin. Invest. 115, 2679-88. 2 . Codogno, P. and Meijer, A.J. (2005) Cell Death Differ. 12 Suppl 2, 1509-18. 3 . Levine, B. and Yuan, J. (2005) J. Clin. Invest. 115, 2679-88. 4 . Corvera, S. (2001) Traffic 2, 859-66. 5 . Stack, J.H. et al. (1995) J Cell Biol 129, 321-34. 6 . Liang, C. et al. (2008) Nat Cell Biol 10, 776-87. 7 . Matsunaga, K. et al. (2009) Nat Cell Biol 11, 385-96. 8 . Zhong, Y. et al. (2009) Nat Cell Biol 11, 468-76. 9 . Sun, Q. et al. (2008) Proc Natl Acad Sci U S A 105, 19211-6. 10 . Itakura, E. et al. (2008) Mol Biol Cell 19, 5360-72. 11 . Liang, C. et al. (2006) Nat Cell Biol 8, 688-99. 12 . Liang, C. et al. (2008) Nat Cell Biol 10, 776-87. 13 . Ionov, Y. et al. (2004) Oncogene 23, 639-45. 14 . Kim, M.S. et al. (2008) Hum Pathol 39, 1059-63. |
Western blot analysis of extracts from various cell lines using UVRAG Antibody (upper) or β-Tubulin (9F3) Rabbit mAb #2128 (lower). HCT 116 cells have been reported to contain a monoallelic frameshift mutation resulting in significantly reduced levels of endogenous UVRAG expression (Ref. 11). Western blot 分析多种细胞系的细胞提取物，使用抗体是UVRAG Antibody (上图) 或 β-Tubulin (9F3) Rabbit mAb 兔单抗#2128 (下图)。据报道，HCT116细胞含有一个单等位基因的移码突变，导致其显著诱导内源性的UVRAG的表达(Ref. 11)。 | |
Western blot analysis of extracts from 293T cells, mock transfected or transfected with human or mouse UVRAG cDNA constructs, using UVRAG Antibody. Western blot 分析293T细胞的细胞提取物，对照转染或转染人源或鼠源UVRAG cDNA质粒，使用抗体是UVRAG Antibody。 |
