| 货号 | 2651S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | USP4 Antibody recognizes endogenous levels of total USP4 protein. Based upon sequence alignment, this antibody is predicted to cross-react with the UnpEL and UnpES isoforms of USP4. This antibody does not cross-react with USP15. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme (DUB) action (1,2). Five DUB subfamilies are recognized, including the USP, UCH, OTU, MJD, and JAMM enzymes. USP4 was originally identified during a survey of murine genes near the Mpv20 retroviral insertion site and intially referred to as Ubiquitous Nuclear Protein (UNP). Analysis of the mouse cDNA originally identified Usp4/Unp as a proto-oncogene related to the human tre-2/tre-17/USP6 proto-oncogene (3,4). Usp4/Unp was subsequently observed to contain the conserved Cys and His boxes of the UBP family (5,6) as well as DUB activity (7,8). In a study of primary lung tumor tissue, it was observed that the human homolog of Usp4, USP4/UNPH, had elevated gene expression levels in small cell tumors and adenocarcinomas of the lung, suggesting a causative role for USP4 in neoplasia (6). Another recent study demonstrated overexpression of USP4 in several types of human cancer and that USP4 positively contributes to cell transformation by negatively regulating p53 levels (9). Both murine and human USP4 have been shown to interact with the Rb family of tumor suppressor proteins, providing additional mechanistic evidence of a role for USP4 in cellular transformation (10, 11). |
| 存放说明 | -20C |
| 计算分子量 | 104, 109 |
| 参考文献 | 1 . Nijman, S.M. et al. (2005) Cell 123, 773-86. 2 . Nalepa, G. et al. (2006) Nat Rev Drug Discov 5, 596-613. 3 . Gupta, K. et al. (1994) Oncogene 9, 1729-31. 4 . Gupta, K. et al. (1993) Oncogene 8, 2307-10. 5 . Papa, F.R. and Hochstrasser, M. (1993) Nature 366, 313-9. 6 . Gray, D.A. et al. (1995) Oncogene 10, 2179-83. 7 . Gilchrist, C.A. et al. (1997) J Biol Chem 272, 32280-5. 8 . Gilchrist, C.A. and Baker, R.T. (2000) Biochim Biophys Acta 1481, 297-309. 9 . Zhang, X. et al. (2011) EMBO J 30, 2177-89. 10 . Blanchette, P. et al. (2001) Oncogene 20, 5533-7. 11 . DeSalle, L.M. et al. (2001) Oncogene 20, 5538-42. |
Western blot analysis of extracts from various cell lines using USP4 Antibody.Western blot检测多种细胞提取物，采用USP4 Antibody。 | |
Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected with a Myc/DDK-tagged cDNA expression construct encoding full-length human USP4, isoform1/UnpEL (hUSP4-Myc/DDK; +), using USP4 Antibody.Western blot检测mock transfected (-)或转染表达Myc/DDK-tagged cDNA编码人全长USP4isoform1/UnpEL (hUSP4-Myc/DDK; +)的293T细胞，采用 USP4 Antibody。 |
