| 货号 | 9435S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Upf1 Antibody detects endogenous levels of total Upf1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Upf1 was identified as an active component in nonsense-mediated decay (NMD), an mRNA surveillance mechanism in eukaryotic cells that degrades mRNAs containing premature termination codons (1). Upf1 was found to be an ATP-dependent RNA helicase in the cytoplasm (2) and was later shown to be a component in the cytoplasmic P-bodies (3). Upf1 phosphorylation mediates translation repression that accompanies NMD, allowing mRNA accessibility to the NMD machinery (4). Two other active components of NMD, Upf2 and Upf3, were also identified (5). Upf2 is perinuclear and Upf3 is nucleocytoplasmic, and both of these proteins play important roles in NMD (5).Upf1蛋白被确认是无义介导的降解（NMD)中的一个活性组分，NMD是真核细胞中mRNA的一种监控机制，能够降解含有提前终止密码子的mRNA(1)。研究发现，Upf1蛋白是胞质中一个ATP依赖的RNA解旋酶(2)，后来发现它还是细胞质加工小体（cytoplasmic P-bodies）中的一个成分(3)。Upf1蛋白的磷酸化能够介导翻译抑制，其伴随着NMD作用，因而允许mRNA更易进入NMD机制(4)。此外，还鉴定出了NMD的另外两个活化分子，Upf2和Upf3。分别定位于核周以及核质(5)。 |
| 存放说明 | -20C |
| 计算分子量 | 140 |
| 参考文献 | 1 . Leeds, P. et al. (1991) Genes Dev 5, 2303-14. 2 . Weng, Y. et al. (1996) Mol Cell Biol 16, 5477-90. 3 . Bruno, I. and Wilkinson, M.F. (2006) Cell 125, 1036-8. 4 . Isken, O. et al. (2008) Cell 133, 314-27. 5 . Lykke-Andersen, J. et al. (2000) Cell 103, 1121-31. |
Western blot analysis of extracts from various cell types using Upf1 Antibody. 使用Upf1 Antibody，Western blot分析不同细胞系中Upf1蛋白水平。 |
