| 货号 | 13789S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | UFD1 Antibody recognizes endogenous levels of total UFD1 protein. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The ubiquitin fusion degradation 1 (UFD1) adaptor protein is a component of a protein complex essential for degradation of misfolded proteins by the endoplasmic reticulum-associated protein degradation (ERAD) pathway (1). The UFD1 protein contains a pair of conserved, amino-terminal ubiquitin-binding sites responsible for binding mono- and polyubiquitin molecules (2,3). The carboxy-terminal region of UFD1 contains binding sites for both the adapter protein NPL4 and the AAA ATPase VCP (4). The UFD1-NPL4 heterodimer binds VCP to create a protein complex responsible for export of misfolded proteins from the ER to the cytoplasm for ubiquitin-mediated degradation (5-7). The same protein complex may also be involved in disassembly of the spindle apparatus following mitosis (8). |
| 存放说明 | -20C |
| 计算分子量 | 40 |
| 参考文献 | 1 . Ye, Y. et al. (2001) Nature 414, 652-6. 2 . Ye, Y. et al. (2003) J Cell Biol 162, 71-84. 3 . Park, S. et al. (2005) Structure 13, 995-1005. 4 . Bruderer, R.M. et al. (2004) J Biol Chem 279, 49609-16. 5 . Meyer, H.H. et al. (2000) EMBO J 19, 2181-92. 6 . Meyer, H.H. et al. (2002) EMBO J 21, 5645-52. 7 . Pye, V.E. et al. (2007) Proc Natl Acad Sci U S A 104, 467-72. 8 . Cao, K. and Zheng, Y. (2004) Cell Cycle 3, 422-4. |
Western blot analysis of extracts from various cell lines using UFD1 Antibody. | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human UFD1 protein (hUFD1-Myc/DDK; +), using UFD1 Antibody. | |
Immunoprecipitation of UFD1 from 293T cell extracts using Normal Rabbit IgG #2729 (lane 2) or UFD1 Antibody (lane 3) Lane 1 is 10% input. Western blot analysis was performed using UFD1 Antibody. |
