| 货号 | 13585S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | UBLE1A/SAE1 Antibody recognizes endogenous levels of total UBLE1A/SAE1 protein. This antibody does not cross-react with NAE1 or UBE1 proteins. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The process of SUMO conjugation to target proteins is similar to the molecular chain of events observed with ubiquitin (1). SUMO is conjugated to target proteins through the coordinated action of the cellular SUMO conjugation machinery, which consists of the E1, E2, and E3 enzymes (2). The canonical SUMO E1 activating enzyme is a heterodimer consisting of Ubiquitin-like 1-activating enzyme E1A (UBLE1A, SAE1) and UBLE1B (SAE2, UBA2) subunits. Mature SUMO is activated by E1 in an ATP-dependent reaction that generates adenylated SUMO, which functions as a high-energy intermediate in the formation of a thioester linkage between SUMO and Cys173 of SAE2 (3,4). SUMO is subsequently transferred from SAE2 to the SUMO E2 conjugating enzyme UBE2I (5). Research studies indicate that UBLE1A (SAE1) is a nuclear protein and c-Myc transcriptional target whose expression is required for Myc-driven tumorigenesis (6-8). |
| 存放说明 | -20C |
| 计算分子量 | 40 |
| 参考文献 | 1 . Geiss-Friedlander, R. and Melchior, F. (2007) Nat Rev Mol Cell Biol 8, 947-56. 2 . Tatham, M.H. et al. (2003) Biochemistry 42, 9959-69. 3 . Desterro, J.M. et al. (1999) J Biol Chem 274, 10618-24. 4 . Gong, L. et al. (1999) FEBS Lett 448, 185-9. 5 . Desterro, J.M. et al. (1997) FEBS Lett 417, 297-300. 6 . Moutty, M.C. et al. (2011) Mol Biol Cell 22, 652-60. 7 . Amente, S. et al. (2012) Am J Cancer Res 2, 330-4. 8 . Kessler, J.D. et al. (2012) Science 335, 348-53. |
Western blot analysis of extracts from various cell lines using UBLE1A/SAE1 Antibody.Western blot检测多种细胞提取物，使用的抗体是UBLE1A/SAE1 Antibody。 | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs encoding Myc/DDK-tagged full-length human SAE1 protein (hSAE1-Myc/DDK; +), Myc/DDK-tagged full-length human NAE1 protein (hNAE1-Myc/DDK; +), or Myc/DDK-tagged full-length human UBE1 protein (hUBE1-Myc/DDK; +), using UBLE1A/SAE1 Antibody (upper) or DYKDDDK Tag Antibody #2368 (lower).Western blot分析293T细胞提取物，对照未转染（-）或转染了Myc/DDK标签的全长人源的SAE1蛋白(hSAE1-Myc/DDK; +)，Myc/DDK标签的全长人源的NAE1蛋白(hNAE1-Myc/DDK; +), 或Myc/DDK标签的全长人源的UBE1蛋白(hUBE1-Myc/DDK; +),使用的抗体是UBLE1A/SAE1 Antibody (上图)或DYKDDDK Tag Antibody #2368 (下图)。 | |
Immunoprecipitation of UBLE1A/SAE1 from MCF7 cell extracts using Normal Rabbit IgG #2729 (lane 2) or UBLE1A/SAE1 Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using UBLE1A/SAE1 Antibody.对MCF7细胞提取物中的UBLE1A/SAE1进行免疫共沉淀，使用抗体是Normal Rabbit IgG #2729 (泳道2)或UBLE1A/SAE1 Antibody (泳道3)。泳道1为10%的上样。Western blot分析使用抗体是UBLE1A/SAE1 Antibody。 |
