| 货号 | 3933S |
| 反应种属 | All |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P |
| 目标/特异性 | Ubiquitin Antibody detects ubiquitin, polyubiquitin and ubiquitinated proteins. This antibody may cross-react with recombinant NEDD8. |
| 使用方法 | WB(1:1000) IHC-P (1:600) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | 泛素是一个在泛素——蛋白酶体途径中发挥重要作用的保守多肽。泛素能够通过泛素转移过程被共价修饰于多个细胞蛋白，导致蛋白通过26S蛋白酶体降解。三个泛素化酶参与泛素偶联过程：泛素首先与活化酶E1形成硫酯键复合体而被激活，然后被转移到泛素载体蛋白E2，然后由E2转移到E3并连接到靶蛋白赖氨酸的epsilon位氨基上(1-3)。泛素——蛋白酶体途径参与多种生物过程和疾病发生，多种信号通路蛋白如IκB、p53、cdc25a 和Bcl-2都受到泛素——蛋白酶体途径调节，影响细胞周期进程、分化、细胞应激和凋亡等过程（4-7）。 |
| 存放说明 | -20C |
| 参考文献 | 1 . Ciechanover, A. (1998) EMBO J 17, 7151-60. 2 . Hochstrasser, M. (2000) Nat Cell Biol 2, E153-7. 3 . Hochstrasser, M. (2000) Science 289, 563-4. 4 . Bernardi, R. et al. (2000) Oncogene 19, 2447-54. 5 . Aberle, H. et al. (1997) EMBO J 16, 3797-804. 6 . Salomoni, P. and Pandolfi, P.P. (2002) Nat Cell Biol 4, E152-3. 7 . Jesenberger, V. and Jentsch, S. (2002) Nat Rev Mol Cell Biol 3, 112-21. |
Western blot analysis of extracts from HeLa and NIH/3T3 cells, untreated or treated with the proteasome inhibitor MG132 (10 µM for 90 minutes), using Ubiquitin Antibody. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Ubiquitin Antibody in the presence of control peptide (left) or antigen-specific peptide (right). | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Ubiquitin Antibody. |
