| 货号 | 3255S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | TTK Antibody detects endogenous levels of total TTK protein. TTK, also known as PYT (Phosphotyrosine Picked Threonine Kinase), is the human homolog of MSP1 (Monopolar Spindle 1) in budding yeast and murine ESK (Embryonal Carcinoma STY Kinase). |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | TTK (Mps1, PYT) is a cell cycle regulated dual specificity kinase present in rapidly proliferating tissues and cell lines (1-3). TTK localizes to kinetochores and centromeres and is an essential component of the mitotic spindle checkpoint as well as centrosome duplication (4-6). The mitotic checkpoint inhibits entry into anaphase until all chromosomes are attached to the spindle; inhibition of this process leads to genomic instability and tumorigenesis. Phosphorylation of the BLM helicase at Ser144 by TTK maintains chromosome stability during mitosis (7). Small molecule inhibitors of TTK can block the spindle checkpoint response, thereby making TTK a potential therapeutic target (8,9). TTK also participates in the DNA damage response by directly phosphorylating and activating the cell cycle checkpoint kinase Chk2 at Thr68. Two targets phosphorylated by Chk2 are the cell cycle phosphatase cdc25 and the transcription factor p53. Inactivation of cdc25 phosphatase results in the accumulation of inactive cyclin B and cell cycle arrest following DNA damage. Phosphorylation of p53 by active Chk2 stabilizes the transcription factor and promotes cell cycle arrest and apoptosis in response to DNA damage (10).TTK（MPS1，PYT）是一种细胞周期调控的双特异性激酶，存在于快速增殖的组织和细胞系（1-3）。TTK集中在着丝粒和中心体，是有丝分裂纺锤体检验点和中心体复制的必不可少的成分(4-6)。有丝分裂检查点抑制后期的进入，直到所有的染色体进入纺锤体；抑制这一过程将导致基因组不稳定和肿瘤的发生。TTK介导的BLM第144位丝氨酸磷酸化能够保持有丝分裂染色体的稳定性(7)。TTK的小分子抑制剂能够阻遏纺锤体检验点反应，由此使TTK成为一个潜在的治疗靶标(8,9)。TTK也参与DNA损伤反应，通过直接磷酸化并激活细胞周期检查点激酶Chk2的第68位苏氨酸。Chk2的两个靶磷酸化靶标分别是细胞周期磷酸酶cdc25和转录因子p53。Cdc25磷酸酶的失活将导致周期蛋白B的累积和伴随DNA损伤的细胞周期捕获。活化的Chk2磷酸化p53能够稳定转录因子并促进细胞周期捕获和凋亡来应答DNA损伤(10)。 |
| 存放说明 | -20C |
| 计算分子量 | 95 |
| 参考文献 | 1 . Mills, G.B. et al. (1992) J. Biol. Chem. 267, 16000-16006. 2 . Stucke, V.M. et al. (2002) EMBO J. 21, 1723-1732. 3 . Lindberg, R.A. et al. (1993) Oncogene 8, 351-359. 4 . Fisk, H.A. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 14875-14880. 5 . Dou, Z. et al. (2003) Cell Res. 13, 443-449. 6 . Abrieu, A. et al. (2001) Cell 106, 83-93. 7 . Leng, M. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 11485-11490. 8 . Schmidt, M. et al. (2005) EMBO Rep. 6, 866-872. 9 . Dorer, R.K. et al. (2005) Curr. Biol. 15, 1070-1076. 10 . Wei, J.H. et al. (2005) J. Biol. Chem. 280, 7748-7757. |
Western blot analysis of recombinant GST-TTK (#7655) and extracts from OVCAR8 and K562 cell lines using TTK Antibody.Western blot方法检测重组GST-TTK (#7655)和OVCAR8和K562细胞提取物，使用的抗体为TTK Antibody。 |
