| 货号 | 3966S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | TRRAP (D2966) Antibody detects endogenous levels of total TRRAP protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Transformation/transcription domain-associated protein (TRRAP) is a highly conserved 434 kDa protein found in various multiprotein complexes, such as SAGA, PCAF, NuA4 and TIP60, which contain histone acetyltransferase (HAT) activity (1-4). TRRAP functions as an adaptor protein by binding directly to the transactivation domains of transcriptional activator proteins and facilitating the recruitment of HAT complexes to acetylate histone proteins and activate transcription (1-5). TRRAP is required for the transcriptional activation and cell transformation activities of c-Myc, E2F1, E2F4, p53 and the adenovirus E1A proteins (1,6,7). TRRAP is also essential in early development and is required at the mitotic checkpoint and for normal cell cycle progression (8,9). In addition, TRRAP has been shown to function in DNA repair. As part of the TIP60 complex, TRRAP is required for the acetylation of histone H4 at double-stranded DNA breaks and subsequent DNA repair by homologous recombination (10). In addition, TRRAP associates with the MRN (MRE11, RAD50, NBS1) complex, which lacks intrinsic HAT activity yet functions in the sensing and subsequent repair of double-stranded breaks by non-homologous DNA end-joining (11). TRRAP shows significant homology to the PI-3 kinase domain of the ATM family of kinases; however, amino acids that map to the catalytic site of the kinase domain are not conserved in TRRAP (1). |
| 存放说明 | -20C |
| 计算分子量 | 434 |
| 参考文献 | 1 . McMahon, S.B. et al. (1998) Cell 94, 363-374. 2 . Grant, P.A. et al. (1998) Mol. Cell 2, 863-867. 3 . Ikura, T. et al. (2000) Cell 102, 463-473. 4 . Allard, S. et al. (1999) EMBO J. 18, 5108-5119. 5 . McMahon, S.B. et al. (2000) Mol. Cell. Biol. 20, 556-562. 6 . Deleu, L. et al. (2001) Oncogene 20, 8270-8275. 7 . Ard, P.G. et al. (2002) Mol. Cell. Biol. 22, 5650-5661. 8 . Herceg, Z. et al. (2001) Nat. Genet. 29, 206-211. 9 . Li, H. et al. (2004) EMBO J. 23, 4824-4834. 10 . Murr, R. et al. (2006) Nat. Cell Biol. 8, 91-99. 11 . Robert, F. et al. (2006) Mol. Cell. Biol. 26, 402-412. |
Western blot analysis of extracts from various cell lines using TRRAP (D2966) Antibody. 使用TRRAP (D2966) Antibody，免疫印迹(Western blot)分析不同细胞中TRRAP (D2966)的蛋白水平。 |
