| 货号 | 5593S |
| 反应种属 | Human/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Troponin T (Cardiac) Antibody detects endogenous levels of total cardiac Troponin T protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits: the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI) (1). In response to β-adrenergic stimulation of the heart, Ser23 and Ser24 of TnI (cardiac) are phosphorylated by PKA and PKC. This phosphorylation stimulates a conformational change of the regulatory domain of TnC, reduces the association between TnI and TnC, and decreases myofilament Ca2+ sensitivity by reducing the Ca2+ binding affinity of TnC (1-3). |
| 存放说明 | -20C |
| 计算分子量 | 40 |
| 参考文献 | 1 . Ward, D.G. et al. (2002) J Biol Chem 277, 41795-801. 2 . Noland, T.A. et al. (1995) J. Biol. Chem. 270, 25445-25454. 3 . Gaponenko, V. et al. (1999) J. Biol. Chem. 274, 16681-16684. |
Western blot analysis of extracts from human and rat heart and human skeletal muscle using Troponin T (Cardiac) Antibody. 使用Troponin T (Cardiac) Antibody，免疫印迹（Western Blot）分析人源和大鼠心脏和人源骨骼肌组织中Troponin T蛋白水平。 | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a human cardiac Troponin T (isoform 1) construct (+), using Troponin T (Cardiac) Antibody. 使用Troponin T (Cardiac) Antibody，免疫印迹（Western Blot）分析293T细胞中Troponin T蛋白水平。细胞分为mock transfected (-)或转染人源心脏的Troponin T (isoform 1) construct (+)。 |
