| 货号 | 2505S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | TrkA Antibody detects endogenous levels of total TrkA protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The family of Trk receptor tyrosine kinases consists of TrkA, TrkB and TrkC. While the sequence of these family members is highly conserved, they are activated by different neurotrophins: TrkA by NGF, TrkB by BDNF or NT4, and TrkC by NT3. TrkA regulates proliferation and is important for development and maturation of the nervous system (1). Phosphorylation at Tyr490 is required for Shc association and activation of the Ras-MAP kinase cascade. Residues Tyr674/675 lie within the catalytic domain, and phosphorylation at this site reflects TrkA kinase activity (2-6). Point mutations, deletions and chromosomal rearrangements (chimeras) cause ligand-independent receptor dimerization and activation of TrkA. Many malignancies including breast, colon, prostate and thyroid carcinomas and acute myeloid leukemia have activated TrkA. Expression of TrkA in neuroblastomas is a good prognostic marker because it signals growth arrest and differentiation of cells originating from the neural crest (1). |
| 存放说明 | -20C |
| 计算分子量 | 140 |
| 参考文献 | 1 . Huang, E.J. and Reichardt, L.F. (2003) Annu Rev Biochem 72, 609-42. 2 . Segal, R.A. and Greenberg, M.E. (1996) Annu Rev Neurosci 19, 463-89. 3 . Stephens, R.M. et al. (1994) Neuron 12, 691-705. 4 . Marsh, H.N. et al. (2003) J Cell Biol 163, 999-1010. 5 . Obermeier, A. et al. (1993) EMBO J 12, 933-41. 6 . Obermeier, A. et al. (1994) EMBO J 13, 1585-90. 7 . Arevalo, J.C. et al. (2001) Oncogene 20, 1229-34. 8 . Reuther, G.W. et al. (2000) Mol Cell Biol 20, 8655-66. 9 . Greco, A. et al. (1997) Genes Chromosomes Cancer 19, 112-23. 10 . Pierotti, M.A. and Greco, A. (2006) Cancer Lett 232, 90-8. 11 . Lagadec, C. et al. (2009) Oncogene 28, 1960-70. 12 . Greco, A. et al. (2010) Mol Cell Endocrinol 321, 44-9. 13 . Ødegaard, E. et al. (2007) Hum Pathol 38, 140-6. |
Western blot analysis from various cell lysates and rat brain cell extracts, using TrkA Antibody. Western blot分析多种细胞和大鼠脑提取物，使用的抗体是TrkA Antibody 兔多抗。 |
