| 货号 | 14981S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | TPP2 Antibody recognizes endogenous levels of total TPP2 protein. This antibody cross-reacts with a 35 kDa protein of unknown identity in some lysates. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Tripeptidyl-peptidase 2 (TPP2) is a well-conserved subtilisin-like amino peptidase that is expressed predominantly in the cytoplasmic compartment (1,2). The amino-terminal region of TPP2 harbors a catalytic triad that is characteristic of serine proteases and allows for TPP2 cleavage of tripeptides from the free amino terminus of oligopeptide substrates (3). TPP2 is a large (>5MDa) homooligomeric protease in which proteolytic activity is regulated by subunit oligomerization (4,5). While TPP2 plays a general role in amino acid homeostasis, research studies demonstrate that TPP2 is involved in MHC class I antigen presentation (6,7) and DNA-damage repair (8). TPP2 activity is required for the survival of Burkitts lymphoma cells, suggesting a possible role for TPP2 in oncogenesis (9). Additional research studies show that TPP2 proteolytic activity is important for regulating lysosome abundance and glycolytic metabolism and that TPP2 deficiency leads to defects in adaptive immunity, innate immunity, and nervous system development (10). |
| 存放说明 | -20C |
| 计算分子量 | 140 |
| 参考文献 | 1 . Tomkinson, B. (1999) Trends Biochem Sci 24, 355-9. 2 . Tomkinson, B. and Lindås, A.C. (2005) Int J Biochem Cell Biol 37, 1933-7. 3 . Hilbi, H. et al. (2002) Biochim Biophys Acta 1601, 149-54. 4 . Seyit, G. et al. (2006) J Biol Chem 281, 25723-33. 5 . Tomkinson, B. (2000) Arch Biochem Biophys 376, 275-80. 6 . Seifert, U. et al. (2003) Nat Immunol 4, 375-9. 7 . York, I.A. et al. (2006) J Immunol 177, 1434-43. 8 . Preta, G. et al. (2009) Biochem Biophys Res Commun 389, 575-9. 9 . Gavioli, R. et al. (2001) Nat Cell Biol 3, 283-8. 10 . Lu, W. et al. (2014) Cell 159, 1578-90. |
Western blot analysis of extracts from various cell lines using TPP2 Antibody. | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human TPP2 protein (hTPP2-Myc/DDK; +), using TPP2 Antibody. | |
Immunoprecipitation of TPP2 from 293T cell extracts using Normal Rabbit IgG #2729 (lane 2) or TPP2 Antibody (lane 3). Lane 1 is 10% input. Western blot was performed using TPP2 Antibody. |
