| 货号 | 3826S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Torc2 Antibody detects endogenous levels of total Torc2 protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Glucose homeostasis is regulated by hormones and cellular energy status. Elevations of blood glucose during feeding stimulate insulin release from pancreatic β-cells through a glucose sensing pathway. Feeding also stimulates release of gut hormones such as glucagon-like peptide-1 (GLP-1), which further induces insulin release, inhibits glucagon release and promotes β-cell viability. CREB-dependent transcription likely plays a role in both glucose sensing and GLP-1 signaling (1). The protein Torc2 (transducer of regulated CREB activity 2) functions as a CREB co-activator (2,3) and is implicated in mediating the effects of these two pathways (4). In quiescent cells, Torc2 is phosphorylated at Ser171 and becomes sequestered in the cytoplasm via an interaction with 14-3-3 proteins. Glucose and gut hormones lead to the dephosphorylation of Torc2 and its dissociation from 14-3-3 proteins. Dephosphorylated Torc2 enters the nucleus to promote CREB-dependent transcription. Torc2 plays a key role in the regulation of hepatic gluconeogenic gene transcription in response to hormonal and energy signals during fasting (5). Torc2-related proteins Torc1 and Torc3 also act as CREB co-activators (2,3). Torc1, Torc2 and Torc3 associate with the HTLV Tax protein to promote Tax-dependent transcription of HTLV-1 long terminal repeats (6,7). Torc1 is highly phosphorylated at Ser151 in mouse hypothalamic cells under basal conditions (8). When these cells are exposed to cAMP or a calcium activator, Torc1 is dephosphorylated and translocates into the nucleus (8). Torc1 is essential for energy balance and fertility (8). |
| 存放说明 | -20C |
| 计算分子量 | 80 |
| 参考文献 | 1 . Hinke, S.A. et al. (2004) J Physiol 558, 369-80. 2 . Conkright, M.D. et al. (2003) Mol Cell 12, 413-23. 3 . Iourgenko, V. et al. (2003) Proc Natl Acad Sci U S A 100, 12147-52. 4 . Screaton, R.A. et al. (2004) Cell 119, 61-74. 5 . Koo, S.H. et al. (2005) Nature 437, 1109-11. 6 . Koga, H. et al. (2004) J Biol Chem 279, 52978-83. 7 . Siu, Y.T. et al. (2006) J Virol 80, 7052-9. 8 . Altarejos, J.Y. et al. (2008) Nat Med 14, 1112-7. |
Western blot analysis of extracts from 293T cells, using Torc2 Antibody. Wetern blot检测293T细胞提取物，所用抗体为Torc2 Antibody。 |
