| 货号 | 3707S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | TNF-α Antibody detects endogenous levels of mature TNF-α as well TNF-α precursor protein. |
| 使用方法 | WB(1:1000) IP (1:25) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | TNF-α, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein (1,2). Membrane-bound TNF-α is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer (2). Both membrane and soluble forms of TNF-α are biologically active. TNF-α is produced by a variety of immune cells including T cells, B cells, NK cells, and macrophages (1). Cellular response to TNF-α is mediated through interaction with receptors TNF-R1 and TNF-R2 and results in activation of pathways that favor both cell survival and apoptosis depending on the cell type and biological context. Activation of kinase pathways (including JNK, Erk (p44/42), p38 MAPK, and NF-κB) promotes the survival of cells, while TNF-α-mediated activation of caspase-8 leads to programmed cell death (1,2). TNF-α plays a key regulatory role in inflammation and host defense against bacterial infection, notably Mycobacterium tuberculosis (3). The role of TNF-α in autoimmunity is underscored by blocking TNF-α action to treat rheumatoid arthritis and Crohn’s disease (1,2,4). |
| 存放说明 | -20C |
| 计算分子量 | 17 mature. 25 precursor. |
| 参考文献 | 1 . Aggarwal, B.B. (2003) Nat Rev Immunol 3, 745-56. 2 . Hehlgans, T. and Pfeffer, K. (2005) Immunology 115, 1-20. 3 . Lin, P.L. et al. (2007) J Investig Dermatol Symp Proc 12, 22-5. |
Western blot analysis of recombinant Human Tumor Necrosis Factor-α (hTNF-α) #8902 using TNF-α Antibody.Western免疫印迹。用TNF-α Antibody研究人源肿瘤坏死因子α(hTNF-α) #8902 的融合蛋白。 | |
Western blot analysis of extracts from Raw 264.7 cells, untreated (-) or LPS-treated (1 µg/ml, 18 hr; +), using TNF-α Antibody. |
