| 货号 | 4125S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | TLK1 Antibody detects endogenous levels of total TLK1. The antibody may cross-react with TLK2. |
| 使用方法 | WB(1:1000) IF-IC (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Tousled-like kinases (TLK1 and TLK2) are nuclear serine/threonine kinases named for their homology to the Tousled gene from Arabidopsis thaliana, essential for flower development (1). The kinase activities of the TLKs are cell cycle regulated, with maximal activity during S phase (1). TLK appears to play a role in chromatin assembly and DNA damage checkpoint regulation (1,2). In C. elegans, TLK1 is essential for appropriate transcription during embryonic development (3). Substrates for TLK include the human chromatin assembly factor Asf, which functions in DNA replication- and repair-coupled chromatin assembly (2). DNA damage during S phase, when TLK is maximally active, leads to inhibition of TLK activity (1). This inhibition requires ataxia mutated kinase (ATM) and Chk1 (4,5). ATM and the related kinase ATR are activited by DNA damage during S phase, phosphorylate Chk1/Chk2, and block the transition into mitosis (6). Chk1 phosphorylates TLK1 on Ser695 in vitro and in vivo, leading to inhibition of TLK1 activity (4). This process likely provides a mechanism to slow the chromatin assembly processes controlled by TLK in the event of DNA damage.Tousled样激酶(TLK1 and TLK2)属于核丝氨酸/苏氨酸激酶，因其与来自拟南芥的Tousled基因的同源性而得名，对于花的发育必不可少(1)。TLKs的激酶活性是细胞周期调控的，在S期活性最高(1)。TLK似乎在染色质装配和DNA损伤检验点调控中起重要作用(1,2)。TLK1对于线虫胚胎发育过程中的适当转录是必需的(3)。TLK的底物包括人类染色质装配因子Asf，它在DNA复制和修复偶联染色质装配过程中起作用(2)。S期DNA损伤时，TLK被最大程度的活化，导致TLK活性抑制(1)。这种抑制需要ATM和Chk1(4,5)。S期DNA损伤激活ATM和相关激酶ATR，使Chk1/Chk2磷酸化，阻遏有丝分裂期的转换(6)。Chk1体内/外都可以磷酸化TLK1的695位丝氨酸，引起TLK1的活性抑制(4)。该过程很可能提供了一种在DNA损伤事件中减慢TLK控制的染色质装配过程。 |
| 存放说明 | -20C |
| 计算分子量 | 86 |
| 参考文献 | 1 . Silljé, H.H. et al. (1999) EMBO J 18, 5691-702. 2 . Silljé, H.H. and Nigg, E.A. (2001) Curr Biol 11, 1068-73. 3 . Han, Z. et al. (2003) Curr Biol 13, 1921-9. 4 . Groth, A. et al. (2003) EMBO J 22, 1676-87. 5 . Krause, D.R. et al. (2003) Oncogene 22, 5927-37. 6 . Kastan, M.B. and Lim, D.S. (2000) Nat. Rev. Mol. Cell Biol. 1, 179-186. |
Western blot analysis of extracts from HeLa (human), C6 (rat), and RAW 264.7 (mouse) cells, using TLK1 Antibody.Western blot 方法检测人Hela细胞，大鼠C6细胞，和小鼠RAW 264.7提取物，使用的抗体为TLK1 Antibody。 | |
Confocal immunofluorescent analysis of HeLa cells using TLK1 Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦免疫荧光方法检测Hela细胞，使用的抗体为 TLK1 Antibody ，呈绿色。肌动蛋白纤维由DY-554鬼笔环肽标记，呈红色。蓝色伪彩为DNA荧光染料，产品信息DRAQ5® #4084。 |
