| 货号 | 5137S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | TIAR Antibody detects endogenous levels of total TIAR protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | TIAR is a member of the RNA-recognition motif (RRM) family of RNA-binding proteins (1,2). It functions as a translational repressor under conditions of cellular damage (3,4). In response to cellular stress, TIAR associates with eIF1, eIF3, and the 40S ribosomal subunit and forms noncanonical preinitiation complexes that are translationally inactive (3,4). TIAR then aggregates with its family member TIA1 and facilitates the accumulation of the translationally inactive preinitiation complexes into discrete cytoplasmic foci called stress granules. The two major isoforms of TIAR are the products of alternative mRNA splicing (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 42 |
| 参考文献 | 1 . Tian, Q. et al. (1991) Cell 67, 629-39. 2 . Kawakami, A. et al. (1992) Proc Natl Acad Sci U S A 89, 8681-5. 3 . Anderson, P. and Kedersha, N. (2002) Cell Stress Chaperones 7, 213-21. 4 . Anderson, P. and Kedersha, N. (2002) J Cell Sci 115, 3227-34. 5 . Kawakami, A. et al. (1994) J Immunol 152, 4937-45. 6 . Beck, A.R. et al. (1996) Nucleic Acids Res 24, 3829-35. |
Western blot analysis of extracts from HeLa and HepG2 cells using TIAR Antibody. 使用TIAR抗体，Western blot分析HeLa和HepG2细胞系中TIAR蛋白水平。 | |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or UV-treated (right), using TIAR Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用TIAR Antibody（绿色）标记，共聚焦免疫荧光观察TIAR蛋白在HeLa细胞中定位，细胞分为对照组 (左图)和UV照射组(右图)。DY-554 phalloidin标记微丝蛋白（红色）。蓝色伪彩= DRAQ5® #4084 (DNA 荧光染料)。 |
