| 货号 | 3766S |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Thymidylate Synthase Antibody detects endogenous levels of total thymidylate synthase protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The methylation of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP) is an essential step in the formation of thymine nucleotides (1,2, reviewed in 3). This process is catalyzed by thymidylate synthase (TS or TYMS), a homodimer composed of two 30 kDa subunits. TS is an intracellular enzyme that provides the sole de novo source of thymidylate, making it a required enzyme in DNA biosynthesis with activity highest in proliferating cells (1). Being the exclusive source of dTMP, TS is also an important target for anticancer agents such as 5-fluorouracil (5-FU) (1-5). 5-FU acts as a TS inhibitor and is active against solid tumors such as colon, breast, head, and neck. Studies have shown that patients with metastases expressing lower levels of TS have a higher response rate to treatment with 5-FU than patients with tumors that have increased levels of TS (5). TS expression may be useful in predicting overall patient survival, but the prognostic value of TS expression continues to be a subject of investigation for different types of cancers (6-10). |
| 存放说明 | -20C |
| 计算分子量 | 30 |
| 参考文献 | 1 . Johnston, P.G. et al. (1991) Cancer Res 51, 6668-76. 2 . Aschele, C. et al. (2002) Ann Oncol 13, 1882-92. 3 . Jackman, A.L. and Calvert, A.H. (1995) Ann Oncol 6, 871-81. 4 . Van Triest, B. et al. (2000) J Histochem Cytochem 48, 755-60. 5 . Johnston, P.G. et al. (1994) J Clin Oncol 12, 2640-7. 6 . Kwon, H.C. et al. (2007) Ann Oncol 18, 504-9. 7 . Allegra, C.J. et al. (2002) J Clin Oncol 20, 1735-43. 8 . Allegra, C.J. et al. (2003) J Clin Oncol 21, 241-50. 9 . Tsourouflis, G. et al. (2008) Dig Dis Sci 53, 1289-96. 10 . Kim, S.H. et al. (2009) Am J Clin Oncol 32, 38-43. |
Western blot analysis of extracts from various cell types using Thymidylate Synthase Antibody. Wetern blot检测多种细胞提取物，所用抗体为Thymidylate Synthase Antibody。 |
