| 货号 | 2519S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | TGF-β Receptor III Antibody detects endogenous levels of the type III TGF-β receptor. This antibody does not cross-react with other family members at physiological conditions. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Transforming growth factor-β (TGF-β) superfamily members are critical regulators of cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis (1-4). TGF-β elicits signaling through three cell surface receptors: type I (RI), type II (RII) and type III (RIII). Type I and type II receptors are serine/threonine kinases that form a heteromeric complex. In response to ligand binding, the type II receptors form a stable complex with the type I receptors allowing phosphorylation and activation of type I receptor kinases (5). The type III receptor, also known as betaglycan, is a transmembrane proteoglycan with a large extracellular domain that binds TGF-β with high affinity but lacks a cytoplasmic signaling domain (6,7). Expression of the type III receptor can regulate TGF-β signaling through presentation of the ligand to the signaling complex. The only known direct TGF-β signaling effectors are the Smad family proteins, which transduce signals from the cell surface directly to the nucleus to regulate target gene transcription (8,9). |
| 存放说明 | -20C |
| 计算分子量 | 110 |
| 参考文献 | 1 . Massague, J. et al. (2000) Cell 103, 295-309. 2 . Caestecker, M.P. et al. (2000) J. Natl. Cancer Inst. 92, 1388-1402. 3 . Derynck, R. et al. (2001) Nature Genet. 29, 117-129. 4 . Miyazono, K. et al. (2000) Adv. Immunol. 75, 115-157. 5 . Derynck, R. et al. (1997) Biochim. Biophys. Acta. 1333, F105-150. 6 . López-Casillas, F. et al. (1991) Cell 67, 785-795. 7 . Wang, X.F. et al. (1991) Cell 67, 797-805. 8 . Derynck, R. et al. (1998) Cell 95, 737-40. 9 . Massague, J. et al. (2000) Nat. Rev. Mol. Cell Biol. 1, 169-178. 10 . Lopez-Casillas, F. et al. (2003) J. Biol. Chem. 278, 382-390. |
Western blot analysis of extracts from K-562 and C2C12 cells upon differentiation into myoblasts using TGF-β Receptor III Antibody. 使用TGF-β Receptor III 抗体对向肌肉细胞分化后的K-562和C2C12细胞提取物进行western blot分析。 |
