| 货号 | 8080S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | TFIIF-α Antibody recognizes endogenous levels of total TFIIF-α protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | TFIIF is a member of the group of general transcription factors that facilitate the binding of RNA polymerase II (Pol II) to promoter sequences as part of the pre-initiation complex (PIC) (1). TFIIF consists of subunits TFIIF-α (RAP74) and TFIIF-β (RAP30). It is involved in the stabilization of Pol II association with the PIC and selection of the transcription start site during transcription initiation (1,2). In addition to its role in transcription initiation, TFIIF has been shown to stimulate the transcription elongation activity of Pol II as well as dephosphorylation and recycling of Pol II during transcription termination (3-5). |
| 存放说明 | -20C |
| 计算分子量 | 74 |
| 参考文献 | 1 . Thomas, M.C. and Chiang, C.M. (2006) Crit Rev Biochem Mol Biol 41, 105-78. 2 . Hahn, S. (2004) Nat Struct Mol Biol 11, 394-403. 3 . Sims, R.J. et al. (2004) Genes Dev 18, 2437-68. 4 . Zhang, C. and Burton, Z.F. (2004) J Mol Biol 342, 1085-99. 5 . Zawel, L. et al. (1995) Genes Dev 9, 1479-90. |
Western blot analysis of extracts from various cell lines using TFIIF-α Antibody. 使用TFIIF-α Antibody，免疫印迹(Western blot)分析不同细胞系中TFIIF-α的蛋白水平。 |
