| 货号 | 13375S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | TFIIE-α Antibody recognizes endogenous levels of total TFIIE-α protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Transcription initiation factor IIE subunit alpha (TFIIE-α) is part of TFIIE, a general transcription factor made up of paired α and β subunits. These general transcription factors include TFIIA, TFIIB, TFIID, TFIIE, TFIIF, and TFIIH (1-4). Binding of RNA polymerase II (RNAPII) to promoter sequences as part of the pre-initiation complex (PIC) is facilitated by these general transcription factors. These factors also help in selection of the proper transcription start site, DNA unwinding, and RNAPII promoter escape during transcription (1). During the transition from transcription initiation to elongation, TFIIE stimulates the TFIIH kinase and DNA helicase activities, responsible for phosphorylation of the carboxy-terminal domain of the largest RNAPII subunit (POL2RA) and unwinding of promoter DNA for RNAPII promoter escape (1,5-9). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
| 参考文献 | 1 . Thomas, M.C. and Chiang, C.M. (2006) Crit Rev Biochem Mol Biol 41, 105-78. 2 . Ohkuma, Y. et al. (1991) Nature 354, 398-401. 3 . Sumimoto, H. et al. (1991) Nature 354, 401-4. 4 . Peterson, M.G. et al. (1991) Nature 354, 369-73. 5 . Ohkuma, Y. and Roeder, R.G. (1994) Nature 368, 160-3. 6 . Ohkuma, Y. et al. (1995) Mol Cell Biol 15, 4856-66. 7 . Serizawa, H. et al. (1994) J Biol Chem 269, 20750-6. 8 . Larochelle, S. et al. (2012) Nat Struct Mol Biol 19, 1108-15. 9 . Grünberg, S. et al. (2012) Nat Struct Mol Biol 19, 788-96. |
Western blot analysis of extracts from various cell lines using TFIIE-α Antibody.Western blot方法检测不同细胞系的提取物，使用的抗体为 TFIIE-α Antibody。 |
