| 货号 | 4240S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | TFEB Antibody recognizes endogenous levels of total human TFEB protein. |
| 使用方法 | WB(1:1000) IP (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Transcription factor EB (TFEB) is a member of the Myc-related, bHLH leucine-zipper family of transcription factors that drives the expression of a network of genes known as the Coordinated Lysosomal Expression and Regulation (CLEAR) network (1,2). TFEB specifically recognizes and binds regulatory sequences within the CLEAR box (GTCACGTGAC) of lysosomal and autophagy genes, resulting in the up-regulated expression of genes involved in lysosome biogenesis and function, and regulation of autophagy (1,2). TFEB is activated in response to nutrient deprivation, stimulating translocation to the nucleus where it forms homo- or heterooligomers with other members of the microphthalmia transcription factor (MiTF) subfamily and resulting in up-regulation of autophagosomes and lysosomes (3-5). Recently, it has been shown that TFEB is a component of mammalian target of rapamycin (mTOR) complex 1 (mTORC1), which regulates the phosphorylation and nuclear translocation of TFEB in response to cellular starvation and stress (6-9). During normal growth conditions, TFEB is phosphorylated at Ser211 in an mTORC1-dependent manner. Phosphorylation promotes association of TFEB with 14-3-3 family proteins and retention in the cytosol. Inhibition of mTORC1 results in a loss of TFEB phosphorylation, dissociation of the TFEB/14-3-3 complex, and rapid transport of TFEB to the nucleus where it increases transcription of CLEAR and autophagy genes (10). TFEB has also been shown to be activated in a nutrient-dependent manner by p42 MAP kinase (Erk2). TFEB is phosphorylated at Ser142 by Erk2 in response to nutrient deprivation, resulting in nuclear localization and activation, and indicating that pathways other than mTOR contribute to nutrient sensing via TFEB (3). |
| 存放说明 | -20C |
| 计算分子量 | 65-70 |
| 参考文献 | 1 . Sardiello, M. et al. (2009) Science 325, 473-7. 2 . Sardiello, M. and Ballabio, A. (2009) Cell Cycle 8, 4021-2. 3 . Settembre, C. et al. (2011) Science 332, 1429-33. 4 . David, R. (2011) Nat Rev Mol Cell Biol 12, 404. 5 . Cuervo, A.M. (2011) Science 332, 1392-3. 6 . Peña-Llopis, S. et al. (2011) EMBO J 30, 3242-58. 7 . Settembre, C. and Ballabio, A. (2011) Autophagy 7, 1379-81. 8 . Peña-Llopis, S. and Brugarolas, J. (2011) Cell Cycle 10, 3987-8. 9 . Settembre, C. et al. (2012) EMBO J 31, 1095-108. 10 . Martina, J.A. et al. (2012) Autophagy 8, (6):903-14. |
Western blot analysis of extracts from various cell lines using TFEB Antibody (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Western blot方法检测不同细胞的提取物，使用的抗体为 TFEB Antibody (上图) 和 GAPDH (D16H11) XP® Rabbit mAb兔单抗 #5174 (下图)。 | |
Immunoprecipitation of TFEB from COLO 205 cells using TFEB Antibody (lane 2) or Normal Rabbit IgG #2729 (lane 3). Lane 1 is 10% input. 免疫沉淀法检测COLO 205细胞提取物中的 TFEB，使用抗体为 TFEB Antibody （泳道2）或 Normal Rabbit IgG #2729(l泳道3)。泳道1为10% input对照。 | |
