| 货号 | 7495S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | TFAM Antibody recognizes endogenous levels of total TFAM protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | TFAM (Transcription Factor A, Mitochondrial; aka TCF6) is a member of the high-mobility group (HMG) proteins because it contains two HMG boxes. It is a transcription factor for mitochondrial DNA (mtDNA), and enhances mtDNA transcription in a promoter-specific fashion in the presence of mitochondrial RNA polymerase and transcription factor B (1). Because the majority of ATP production depends on the mitochondrial respiratory chain, maintenance of the mitochondrial genome is absolutely critical for individuals to maintain normal health. TFAM plays an essential role in the maintenance of mtDNA and thus, ATP production (2). TFAM binds to mtDNA both nonspecifically and in a sequence-specific manner. It is abundant enough to completely enwrap mtDNA and is known to have a dual effect on mtDNA: protection of mtDNA and initiation of transcription from mtDNA (3). TFAM attenuates age-dependent impairment of the brain by preventing oxidative stress and mitochondrial dysfunctions in microglia (4). |
| 存放说明 | -20C |
| 计算分子量 | 24 |
| 参考文献 | 1 . Kanki, T. et al. (2004) Mol Cell Biol 24, 9823-34. 2 . Kang, D. et al. Mitochondrion 7, 39-44. 3 . Watanabe, A. et al. (2011) Cardiovasc Res, Epub ahead of print. 4 . Hayashi, Y. et al. (2008) J Neurosci 28, 8624-34. |
Western blot analysis of extracts from HeLa and 293T cells using TFAM Antibody. Western blot分析HeLa和293T细胞提取物，使用的抗体是TFAM Antibody 兔多抗。 |
