| 货号 | 3449S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | TDP43 (A260) Antibody detects endogenous levels of total TDP43 protein. |
| 使用方法 | WB(1:1000) IF-IC (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | TDP43 (TAR DNA-binding protein 43) is involved in transcriptional regulation and exon splicing (1,2). While normal TDP43 is a nuclear protein, pathological TDP43 is a component of insoluble aggregates in patients with frontotemporal lobar degeneration (FTLD) and amyotrophic lateral sclerosis (ALS). In these disorders, TDP43 is abnormally ubiquitinated, phosphorylated and cleaved to generate carboxy-terminal fragments that are sequestered as insoluble aggregates in neuronal nuclei, perikarya, and neurites (3,4). Additionally, TDP43 inhibits the expression of the HIV-1 gene and regulates CFTR gene splicing (1,5). |
| 存放说明 | -20C |
| 计算分子量 | 45 |
| 参考文献 | 1 . Ou, S.H. et al. (1995) J Virol 69, 3584-96. 2 . Winton, M.J. et al. (2008) J Biol Chem 283, 13302-9. 3 . Neumann, M. et al. (2006) Science 314, 130-3. 4 . Cairns, N.J. et al. (2007) Am J Pathol 171, 227-40. 5 . Buratti, E. and Baralle, F.E. (2001) J Biol Chem 276, 36337-43. |
Confocal immunofluorescent analysis of HeLa cells using TDP43 (A260) Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). 共聚焦免疫荧光分析HeLa细胞，使用的抗体是TDP43(A260) Antibody 兔多抗（绿色）。肌动蛋白纤丝用DY-554 phalloidin（红色）标记。 | |
Western blot analysis of extracts from HeLa cells and rat brain using TDP43 (A260) Antibody. Western blot分析HeLa细胞和大鼠大脑提取物，使用的抗体是TDP43(A260) Antibody 兔多抗。 |
