| 货号 | 3685S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | TCEB3/Elongin A Antibody recognizes endogenous levels of total TCEB3/Elongin A protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The Elongin complex is a heterotrimer composed of TCEB3/Elongin A, Elongin B (TCEB2), and Elongin C (TCEB1) subunits (1-3). The Elongin complex regulates the rate of RNA polymerase II (RNAPII) transcription elongation by releasing the transient pausing of RNAPII at multiple sites along the DNA. TCEB3/Elongin A is the transcriptionally active subunit, while Elongin B and C subunits play a regulatory role (3,4). TCEB3/Elongin A may be required for expression of a subset of cell cycle regulated genes, and embryonic stem (ES) cells lacking TCEB3/Elongin A show abnormalities in cell size, growth, and cell cycle distribution (5). In addition, the Elongin complex has been shown to interact with the cullin family and RING finger proteins Cul5/Rbx2 upon UV-induced DNA damage, removing arrested RNAPII at sites of DNA damage by ubiquitination and degradation as part of an E3 ubiquitin ligase (6). |
| 存放说明 | -20C |
| 计算分子量 | 100 |
| 参考文献 | 1 . Bradsher, J.N. et al. (1993) J Biol Chem 268, 25587-93. 2 . Garrett, K.P. et al. (1994) Proc Natl Acad Sci U S A 91, 5237-41. 3 . Aso, T. et al. (1995) Science 269, 1439-43. 4 . Aso, T. et al. (1996) EMBO J 15, 5557-66. 5 . Yamazaki, K. et al. (2003) J Biol Chem 278, 13585-9. 6 . Yasukawa, T. et al. (2008) EMBO J 27, 3256-66. |
Western blot analysis of extracts from various cell lines using TCEB3/Elongin A Antibody. 使用TCEB3/Elongin A Antibody，免疫印迹(Western blot)分析不同细胞中TCEB3/Elongin A蛋白水平。 |
