| 货号 | 8515S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | TBP Antibody recognizes endogenous levels of total TBP protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | TATA-binding protein (TBP) is a ubiquitously expressed nuclear protein that functions at the core of the general transcription factor protein complex TFIID (1-3). TFIID, which contains TBP and 13 TBP-associated factors (TAFs), contributes to the formation of the transcription pre-initiation complex, an assembly of multiple protein complexes (TFIIA, TFIIB, TFIIE, TFIIF, TFIIH, and RNA polymerase II) that bind to a gene promoter during the initiation of transcription (1-3). Once the pre-initiation complex is formed, RNA polymerase II becomes competent for elongation and transcribes the body of a gene. TBP functions in the recruitment of TFIID by binding to the TATA-box sequence found approximately 25 base pairs upstream of the transcription start site of many protein-coding genes. In addition, many transcriptional activator proteins interact with TBP and various TAF proteins to facilitate recruitment of TFIID and formation of the pre-initiation complex. |
| 存放说明 | -20C |
| 计算分子量 | 38 |
| 参考文献 | 1 . Goodrich, J.A. and Tjian, R. (1994) Curr Opin Cell Biol 6, 403-9. 2 . Berk, A.J. (2000) Cell 103, 5-8. 3 . Thomas, M.C. and Chiang, C.M. (2006) Crit Rev Biochem Mol Biol 41, 105-78. |
Western blot analysis of extracts from various cell lines using TBP Antibody. 使用TBP Antibody，免疫印迹(Western blot)分析不同细胞中TBP的蛋白水平。 |
