| 货号 | 4629S |
| 反应种属 | Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | TBC1D1 (V796) Antibody detects endogenous levels of total TBC1D1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | TBC1D1 is a paralog of AS160 (1) and both proteins share about 50% identity (2). TBC1D1 was shown to be a candidate gene for severe obesity (3). It plays a role in Glut4 translocation through its GAP activity (2,4). Studies indicate that TBC1D1 is highly expressed in skeletal muscle (1). Insulin, AICAR, and contraction directly regulate TBC1D1 phosphorylation in this tissue (1). Three AMPK phosphorylation sites (Ser231, Ser660, and Ser700) and one Akt phosphorylation site (Thr590) were identified (5). Muscle contraction or AICAR treatment increases phosphorylation on Ser231, Ser660, and Ser700 but not on Thr590; insulin increases phosphorylation on Thr590 only (5). |
| 存放说明 | -20C |
| 计算分子量 | 160 |
| 参考文献 | 1 . Taylor, E.B. et al. (2008) J Biol Chem 283, 9787-96. 2 . Roach, W.G. et al. (2007) Biochem J 403, 353-8. 3 . Stone, S. et al. (2006) Hum Mol Genet 15, 2709-20. 4 . Chavez, J.A. et al. (2008) J Biol Chem 283, 9187-95. 5 . Vichaiwong, K. et al. (2010) Biochem J 431, 311-20. |
Western blot analysis of extracts from C2C12 myotubes using TBC1D1 (V796) Antibody. Wetern blot检测多种细胞系提取物，所用抗体为TBC1D1 (V796) Antibody。 |
