| 货号 | 4883S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/ChIP |
| 目标/特异性 | TAZ (V386) Antibody detects endogenous levels of total TAZ protein. |
| 使用方法 | WB(1:1000) ChIP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | TAZ is a transcriptional co-activator with a PDZ-binding motif that is regulated by its interaction with 14-3-3 proteins (1). TAZ shares homology with the WW domain of Yes-associated protein (YAP) (1). TAZ is proposed to modulate the switch between proliferation and differentiation of mesenchymal stem cells (MSC) via interaction with transcription factors Runx2 and PPARγ. This process is critical to normal tissue development and the prevention of tumor formation. Due to its role in determination of MSC fate, TAZ may have clinical relevance to several human diseases caused by an imbalance of MSC differentiation (2,3). TAZ appears to be negatively regulated by LATS tumor suppressor kinase, a component of the Hippo pathway that controls tissue growth and tumor development (4). |
| 存放说明 | -20C |
| 计算分子量 | 50 |
| 参考文献 | 1 . Kanai, F. et al. (2000) EMBO J 19, 6778-91. 2 . Hong, J.H. et al. (2005) Science 309, 1074-8. 3 . Hong, J.H. and Yaffe, M.B. (2006) Cell Cycle 5, 176-9. 4 . Lei, Q.Y. et al. (2008) Mol Cell Biol 28, 2426-36. |
Western blot analysis of extracts from various cell lines using TAZ (V386) Antibody.使用TAZ (V386) 抗体对多种细胞提取物进行Western blot分析。 | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 NCI-2052 cells and either 10 µl of TAZ (V386) Antibody, or 2 µl of Normal Rabbit IgG #2729, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human CTGF Promoter Primers #14927, human SMYD3 intron 2 primers, and SimpleChIP® Human CTGF Upstream Primers #14928. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
