| 货号 | 12259S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | TAP2 Antibody recognizes endogenous levels of total TAP2 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | CD8+ cytotoxic T cells recognize peptides presented by MHC class I molecules on the surface of infected cells and tumor cells. The transporters associated with antigen processing 1 and 2 (TAP1 and TAP2) form the TAP complex which resides on the ER membrane and transports peptides from the cytoplasm into the ER for loading onto MHC class I molecules (1-8). In addition, TAP localized to endosomal membranes is important for cross-presentation by dendritic cells (9,10). IFN-γ produced by T cells and NK cells in response to infection causes upregulation of TAP1 and TAP2, resulting in increased antigen presentation to T cells (11). Some viral proteins inhibit TAP function or downregulate TAP expression resulting in viral immune evasion (12,13). In addition, investigators have observed reduced TAP expression in a variety of tumor types, and it is thought to be one mechanism for tumor immune evasion (14). |
| 存放说明 | -20C |
| 计算分子量 | 72 |
| 参考文献 | 1 . Trowsdale, J. et al. (1990) Nature 348, 741-4. 2 . Spies, T. et al. (1990) Nature 348, 744-7. 3 . Deverson, E.V. et al. (1990) Nature 348, 738-41. 4 . Monaco, J.J. et al. (1990) Science 250, 1723-6. 5 . Spies, T. and DeMars, R. (1991) Nature 351, 323-4. 6 . Kleijmeer, M.J. et al. (1992) Nature 357, 342-4. 7 . Kelly, A. et al. (1992) Nature 355, 641-4. 8 . Spies, T. et al. (1992) Nature 355, 644-6. 9 . Huang, A.Y. et al. (1996) Immunity 4, 349-55. 10 . Guermonprez, P. et al. (2003) Nature 425, 397-402. 11 . Bahram, S. et al. (1991) Proc Natl Acad Sci U S A 88, 10094-8. 12 . Früh, K. et al. (1995) Nature 375, 415-8. 13 . Bennett, E.M. et al. (1999) J Immunol 162, 5049-52. 14 . Steer, H.J. et al. (2010) Oncogene 29, 6301-13. |
Western blot analysis of extracts from HeLa and HT-29 cells, untreated (-) or treated with Human Interferon-γ (hIFN-γ) #8901 (10 ng/ml, 16 hr; +), using TAP2 Antibody (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).Western blot分析HeLa和HT-29细胞的提取物，未处理(-)或用Human Interferon-γ (hIFN-γ) #8901 (10 ng/ml, 16 小时; +)处理。使用的抗体是：TAP2 Antibody（上图）、β-Actin (D6A8) Rabbit mAb #8457（下图）。 | |
Immunoprecipitation of TAP2 from HeLa cell extracts treated with Human Interferon-γ (hIFN-γ) #8901 (10 ng/ml, 16 hr), using Normal Rabbit IgG #2729 (lane 2) or TAP2 Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using TAP2 Antibody.免疫沉淀分析来源于HeLa细胞提取物的TAP2，用Human Interferon-γ (hIFN-γ) #8901 (10 ng/ml, 16 小时)处理。使用的抗体是：Normal Rabbit IgG #2729（泳道2）、TAP2 Antibody（泳道3）。泳道1是10% input。Western blot分析使用的抗体是TAP2 Antibody。 |
