| 货号 | 14151S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Syntaxin 5 Antibody recognizes endogenous levels of total syntaxin 5 protein. This antibody also cross-reacts with an unidentified protein of 120 kDa. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The membrane protein syntaxin 5 (STX5) is a key component of soluble N-ethylmaleimide-sensitive factor attachment protein (SNAP) receptor (SNARE) complexes that regulate cellular protein transport, vesicle docking, and membrane fusion (1). Syntaxin 5 protein is found as a 42 kDa ("long") protein localized to the Golgi complex and endoplasmic reticulum, and a “short” 35 kDa isoform localized primarily to the Golgi (2,3). Formation of the syntaxin 5 SNARE complex, which also includes proteins Sec22B, Bet1, GOSR1, GOSR2, and Ykt6, allows for regulation of ER-to-Golgi transport, intra-Golgi transport, and endosome-to-Golgi retrograde transport (4-6). Research studies indicate that the syntaxin 5 SNARE complex also plays an essential role in autophagy following autophagosome formation. Intracellular protein transport mediated by the syntaxin 5 complex is required for transport and localized activity of lysosomal proteases. The experimental reduction or deletion of syntaxin 5 complex components results in non-functional lysosomes and accumulation of autophagosomes (7). |
| 存放说明 | -20C |
| 计算分子量 | 35, 42 |
| 参考文献 | 1 . Hong, W. (2005) Biochim Biophys Acta 1744, 120-44. 2 . Volchuk, A. et al. (2004) Mol Biol Cell 15, 1506-18. 3 . Miyazaki, K. et al. (2012) J Cell Sci 125, 5658-66. 4 . Tai, G. et al. (2004) Mol Biol Cell 15, 4011-22. 5 . Xu, Y. et al. (2002) Mol Biol Cell 13, 3493-507. 6 . Zhang, T. and Hong, W. (2001) J Biol Chem 276, 27480-7. 7 . Renna, M. et al. (2011) J Cell Sci 124, 469-82. |
Western blot analysis of extracts from various cell lines using Syntaxin 5 Antibody. |
