| 货号 | 4917S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | STIM2 Antibody detects endogenous levels of total STIM2 protein. |
| 使用方法 | WB(1:1000) IF-IC (1:25) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Ca2+ is a key second messenger in many intracellular signaling pathways. Ca2+ signals control many cellular functions ranging from short-term responses such as contraction and secretion to longer-term regulation of cell growth and proliferation (1,2). Stromal interaction molecules (STIMs) function as Ca2+ sensors that detect changes in Ca2+ content in intracellular Ca2+ stores (3). STIM1 is conserved, ubiquitously expressed, and functions as an endoplasmic reticulum (ER) Ca2+ sensor that migrates from the ER Ca2+ store to the plasma membrane where it activates calcium-release-activated calcium (CRAC) channels when the ER Ca2+ store is low (4). STIM1 is a potential tumor suppressor; defects in STIM1 may cause rhabdomyosarcoma and rhabdoid tumors (5). STIM1 can either homodimerize or form heterodimers with STIM2. STIM2 possesses a high sequence identity to STIM1 and can function as an inhibitor of STIM1-mediated plasma membrane store-operated Ca2+ entry (6). However, further investigation is required to elucidate the true physiological function of STIM2. |
| 存放说明 | -20C |
| 计算分子量 | 100 |
| 参考文献 | 1 . Berridge, M.J. et al. (2000) Nat Rev Mol Cell Biol 1, 11-21. 2 . Berridge, M.J. et al. (2003) Nat Rev Mol Cell Biol 4, 517-29. 3 . Zheng, L. et al. (2008) Biochem Biophys Res Commun 369, 240-6. 4 . Zhang, S.L. et al. (2005) Nature 437, 902-5. 5 . Manji, S.S. et al. (2000) Biochim Biophys Acta 1481, 147-55. 6 . Soboloff, J. et al. (2006) Curr Biol 16, 1465-70. |
Western blot analysis of extracts from various cell lines using STIM2 Antibody. 对多个细胞系使用STIM2抗体进行Western blot分析。 | |
Confocal immunofluorescent analysis of HEK/293 cells using STIM2 Antibody (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 对HEK/293细胞使用STIM2抗体（绿色）进行共聚焦免疫荧光分析。蓝色伪彩=DRAQ5® #4084(荧光DNA染料)。 |
