| 货号 | 3352S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P |
| 目标/特异性 | Stathmin Antibody detects endogenous levels of total stathmin protein. The antibody does not cross-react with related proteins such as SCG10, SCLIP and RB3. |
| 使用方法 | WB(1:1000) IHC-P (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Stathmin is a ubiquitously expressed microtubule destabilizing phosphoprotein that is upregulated in a number of cancers. The amino terminus of the protein contains multiple phosphorylation sites and is involved in the promotion of tubulin filament depolymerization. Phosphorylation at these sites inactivates the protein and stabilizes microtubules. Ser16 phosphorylation by CaM kinases II and IV (1,2) increases during G2/M-phase and is involved in mitotic spindle regulation (3,4). Ser38 is a target for cdc2 kinase (5) and TNF-induced cell death gives rise to reactive oxygen intermediates leading to hyperphosphorylation of stathmin (6). EGF receptor activation of Rac and cdc42 also increases phosphorylation of stathmin on Ser16 and Ser38 (7). Other closely related family members are neuronally expressed and include SCG10, SCLIP, RB3 and its splice variants RB3 and RB3. Stathmin and SCG10 have been shown to play roles in neuronal-like development in PC12 cells (8). |
| 存放说明 | -20C |
| 计算分子量 | 19 |
| 参考文献 | 1 . Marklund, U. et al. (1994) Eur J Biochem 225, 53-60. 2 . le Gouvello, S. et al. (1998) J Immunol 161, 1113-22. 3 . Mistry, S.J. and Atweh, G.F. (2001) J Biol Chem 276, 31209-15. 4 . Gavet, O. et al. (1998) J Cell Sci 111 ( Pt 22), 3333-46. 5 . Luo, X.N. et al. (1994) J Biol Chem 269, 10312-8. 6 . Vancompernolle, K. et al. (2000) J Biol Chem 275, 33876-82. 7 . Daub, H. et al. (2001) J Biol Chem 276, 1677-80. 8 . Di Paolo, G. et al. (1996) J Cell Biol 133, 1383-90. |
Western blot analysis of extracts from C6, SH-SY5Y, SK-N-MC, PC12, COS and HeLa cells, using Stathmin Antibody. 使用Stathmin Antibody，免疫印迹（Western Blot）分析C6、SH-SY5Y、SK-N-MC、PC12、COS和HeLa细胞中Stathmin蛋白水平。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization, using Stathmin Antibody. 使用Stathmin Antibody，免疫组化分析人源乳腺癌组织石蜡切片，结果显示细胞质定位。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Stathmin Antibody. 使用Stathmin Antibody，免疫组化分析人源乳腺癌组织石蜡切片。 |
