| 货号 | 14674S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | STAM2 Antibody recognizes endogenous levels of total STAM2 protein. This antibody does not cross-react with STAM1. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Signal transducing adaptor molecule 2 (STAM2) is a ubiquitously expressed STAM family adaptor protein and an integral component of the ESCRT-0 complex. Similar to STAM1, STAM2 possesses a single SH3 domain and an immunoreceptor tyrosine-based activation motif (ITAM). Following activation of multiple growth factor and cytokine cell surface receptors, the STAM2 protein undergoes tyrosine phosphorylation and potentiates mitogenic signals driven by these receptors (1,2). Research studies demonstrate that STAM2 is localized to complexes containing Eps15, Hrs, and STAM1 proteins on early endosome membranes. A tandem, amino-terminal VHS (Vps27/Hrs/STAM) domain and UIM (ubiquitin-interacting) motif within STAM2 facilitate STAM2 interaction with ubiquitinated cargo proteins, suggesting that this adaptor participates in the endosomal sorting of ubiquitinated proteins targeted for lysosomal degradation (3-6). Gene targeting studies have revealed an indispensible role for STAM2 in T-cell development (7). |
| 存放说明 | -20C |
| 计算分子量 | 70 |
| 参考文献 | 1 . Endo, K. et al. (2000) FEBS Lett 477, 55-61. 2 . Pandey, A. et al. (2000) J Biol Chem 275, 38633-9. 3 . Mizuno, E. et al. (2003) Mol Biol Cell 14, 3675-89. 4 . Bache, K.G. et al. (2003) J Biol Chem 278, 12513-21. 5 . Takata, H. et al. (2000) Genes Cells 5, 57-69. 6 . Mizuno, E. et al. (2004) J Biochem 135, 385-96. 7 . Yamada, M. et al. (2002) Mol Cell Biol 22, 8648-58. |
Western blot analysis of extracts from various cell lines using STAM2 Antibody. | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human STAM1 protein (hSTAM1-Myc/DDK; +) or Myc/DDK-tagged full-length human STAM2 protein (hSTAM2-Myc/DDK; +), using STAM2 Antibody (upper) and DYKDDDDK Tag Antibody #2368 (lower). | |
Immunoprecipitation of STAM2 from 293T cell extracts using Normal Rabbit IgG #2729 (lane 2) or STAM2 Antibody (lane 3). Lane 1 is 10% input. Western blot was performed using STAM2 Antibody. |
