| 货号 | 9033S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | SPT5 Antibody recognizes endogenous levels of total SPT5 protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | DRB-sensitivity inducing factor (DSIF), a heterodimer composed of SPT4 and SPT5, is capable of both facilitating and inhibiting RNA polymerase II (RNAPII) activity (1-3). DSIF, together with NELF (Negative Elongation Factor), inhibits RNAPII elongation, resulting in promoter proximal pausing of RNAPII as it awaits additional signaling to resume transcription (4). The release of promoter proximal pausing is signaled through phosphorylation of the RNAPII C-terminal domain (CTD) and NELF by positive transcription elongation factor (P-TEFb) (5). P-TEFb also phosphorylates SPT5 at Thr4 within the evolutionarily conserved heptapeptide repeat motif. This phosphorylation event switches DSIF from a transcriptional repressor to an activator where it becomes a critical factor for transcriptional elongation (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 150 |
| 参考文献 | 1 . Wada, T. et al. (1998) Genes Dev 12, 343-56. 2 . Saunders, A. et al. (2006) Nat Rev Mol Cell Biol 7, 557-67. 3 . Gomes, N.P. et al. (2006) Genes Dev 20, 601-12. 4 . Yamaguchi, Y. et al. (1999) Cell 97, 41-51. 5 . Buratowski, S. (2009) Mol Cell 36, 541-6. 6 . Wada, T. et al. (1998) EMBO J 17, 7395-403. 7 . Yamada, T. et al. (2006) Mol Cell 21, 227-37. |
Western blot analysis of extracts from various cell lines using SPT5 Antibody. 使用SPT5 Antibody，免疫印迹(Western blot)分析不同细胞中SPT5的蛋白水平。 |
