| 货号 | 5420S |
| 反应种属 | Human/Mouse/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | SPARC Antibody detects endogenous levels of total SPARC protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | SPARC (secreted protein acidic and rich in cysteine), also known as osteonectin and BM40, is a secreted matricellular glycoprotein that belongs to a group of functionally related glycoproteins that includes tenascins C and X, thrombospondins 1 and 2, and osteopontin (1). Members in this class of glycoproteins are involved in tissue renewal, tissue remodeling, and embryonic development and work by exerting counter-adhesive and antiproliferative effects that lead to changes in cell shape, disruption of cell adhesion, and inhibition of the cell cycle (2). SPARC is expressed at high levels in bone tissue but is widely distributed in many other tissues and cell types (3), and is known to be associated with tissues undergoing morphogenesis, angiogenesis, mineralization, and other pathological responses to injury and tumorigenesis (4,5). SPARC has also been linked with obesity and diabetes (6). |
| 存放说明 | -20C |
| 计算分子量 | 42 |
| 参考文献 | 1 . Fukunaga-Kalabis, M. et al. (2008) Cancer Microenviron 1, 93-102. 2 . Yan, Q. and Sage, E.H. (1999) J Histochem Cytochem 47, 1495-506. 3 . Maillard, C. et al. (1992) Bone 13, 257-64. 4 . Sage, E.H. (1997) Nat Med 3, 144-6. 5 . Bradshaw, A.D. and Sage, E.H. (2001) J Clin Invest 107, 1049-54. 6 . Kos, K. and Wilding, J.P. (2010) Nat Rev Endocrinol 6, 225-35. |
Western blot analysis of various cell extracts using SPARC Antibody. Jurkat are negative, as expected.SPARC在western blot实验中检测多种细胞提取物。如预期，Jurkat细胞为阴性。 | |
Confocal immunofluorescent analysis of NTERA-2 (left) and SW620 (right) cells using SPARC Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).SPARC抗体（绿色）对NTERA-2 （左） 和 SW620 （右） 进行激光共聚焦荧光分析。使用DY-554鬼笔环肽（红色）标记肌动蛋白丝。蓝色假色=DRAQ5® #4084 (荧光DNA染料). |
