| 货号 | 2281S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IF-F |
| 目标/特异性 | SPAK Antibody detects endogenous levels of total SPAK protein. This antibody does not cross-react with OSR1 or other members of the GCK family. |
| 使用方法 | WB(1:1000) IF-F (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | SPAK (STE20/SPS1-related Pro/Ala-rich kinase) and OSR1 (oxidative stress responsive 1) are members of the GCK family serine/threonine kinases. Overexpression and in vitro studies demonstrate that SPAK is able to activate p38 MAP kinase indicating a possible role for SPAK in the stress response (1). Yeast two-hybrid screening revealed that SPAK and OSR1 bind to Na-K-2Cl cotransporters NKCC1 and NKCC2 and K-Cl cotransporter KCC3 (2). WNK1 and WNK4 phosphorylate SPAK at Thr243/247 and Ser380 (3-5). Similarly, WNK1 and WNK4 phosphorylate OSR1 at Thr185 and Ser315 (3, 4). Phosphorylation at these sites stimulates SPAK and OSR1 activity, leading to NKCC1 phosphorylation and enhanced NKCC1 activity (3-5). SPAK is also phosphorylated at Ser311 by PKCθ in response to T cell activation. Substitution of Ser311 with Ala or specific siRNA knock-down of SPAK dramatically reduces TCR/CD28-induced AP-1 activation, suggesting SPAK is involved in T cell signaling as well (6). |
| 存放说明 | -20C |
| 计算分子量 | 65 |
| 参考文献 | 1 . Johnston, A.M. et al. (2000) Oncogene 19, 4290-7. 2 . Piechotta, K. et al. (2002) J Biol Chem 277, 50812-9. 3 . Vitari, A.C. et al. (2005) Biochem J 391, 17-24. 4 . Moriguchi, T. et al. (2005) J Biol Chem 280, 42685-93. 5 . Gagnon, K.B. et al. (2006) Mol Cell Biol 26, 689-98. 6 . Li, Y. et al. (2004) EMBO J 23, 1112-22. |
Confocal immunofluorescent analysis of choroid plexus epithelial cells showing apical membrane localization, using SPAK Antibody (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用SPAK Antibody (红色)标记，共聚焦免疫荧光分析choroid plexus epithelial细胞，结果显示定位于顶膜。蓝色伪彩= DRAQ5® #4084（DNA荧光染料）。 | |
Western blot analysis of extracts from 293, C2C12 and NBT-II cells, using SPAK Antibody. 使用SPAK Antibody，免疫印迹（Western Blot）分析293、C2C12和NBT-II细胞中SPAK蛋白水平。 |
