| 货号 | 5931S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | SP1 Antibody detects endogenous levels of total SP1 protein. |
| 使用方法 | WB(1:1000) IP (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Specificity protein 1 (SP1) is a ubiquitously expressed transcription factor belonging to the family of C2H2-type zinc finger containing DNA-binding proteins. SP1 binds GC-rich motifs with high affinity and regulates the expression of numerous mammalian genes (1,2). It interacts with many other transcription factors, such as c-Myc, Egr1, and Stat1, and with basal transcription machinery components. SP1 interacts with chromatin-modifying factors such as histone deacetylases (HDACs) and p300 in chromatin remodeling. Transcriptional activity and stability of SP1 are regulated by post-translational modification, including phosphorylation, acetylation, ubiquitylation and glycosylation (3). Glycosylation of SP1 following insulin treatment leads to increased nuclear localization, while glucagon treatment increases cytoplasmic SP1 levels (4-6). High levels of SP1 have been found in Alzheimer disease (AD) patients, suggesting that SP1 and may be a therapeutic target for the treatment of AD (7). |
| 存放说明 | -20C |
| 计算分子量 | 90 |
| 参考文献 | 1 . Kadonaga, J.T. et al. (1987) Cell 51, 1079-90. 2 . Song, J. et al. (2003) Int J Mol Med 11, 547-53. 3 . Tan, N.Y. and Khachigian, L.M. (2009) Mol Cell Biol 29, 2483-8. 4 . Majumdar, G. et al. (2003) Am J Physiol Endocrinol Metab 285, E584-91. 5 . Majumdar, G. et al. (2006) J Biol Chem 281, 3642-50. 6 . Solomon, S.S. et al. (2008) Life Sci 83, 305-12. 7 . Citron, B.A. et al. (2008) J Neurosci Res 86, 2499-504. |
Western blot analysis of extracts from RL7, SR, and Ramos cells using SP1 Antibody. 使用SP1 Antibody，免疫印迹(Western blot)分析RL7、SR和Ramos细胞中SP1的蛋白水平。 |
