| 货号 | 2748S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/ChIP |
| 目标/特异性 | Sox2 Antibody detects endogenous levels of total Sox2 protein. |
| 使用方法 | WB(1:1000) IP (1:100) ChIP (1:25) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Embryonic stem cells are derived from the inner cell mass of the blastocyst and are unique in their pluripotent capacity and potential for self-renewal. Sox2 is one of a set of transcription factors that are crucial for the maintenance of pluripotency (1). Sox2, Oct-4, and Nanog cooperate in this network (1-3) and siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency (4,5). Chromatin immunoprecipitation experiments have shown that Sox2 and Oct-4 bind to thousands of gene regulatory sites, highlighting the importance of these transcription factors in early embryonic development (6,7). It has recently been shown that Sox2 is amplified in lung and esophageal squamous cell tumors (8). |
| 存放说明 | -20C |
| 计算分子量 | 35 |
| 参考文献 | 1 . Conley, B.J. et al. (2004) Int J Biochem Cell Biol 36, 555-67. 2 . Pesce, M. and Schöler, H.R. (2001) Stem Cells 19, 271-8. 3 . Pan, G. and Thomson, J.A. (2007) Cell Res 17, 42-9. 4 . Boyer, L.A. et al. (2005) Cell 122, 947-56. 5 . Loh, Y.H. et al. (2006) Nat Genet 38, 431-40. 6 . Matin, M.M. et al. (2004) Stem Cells 22, 659-68. 7 . Takahashi, K. and Yamanaka, S. (2006) Cell 126, 663-76. 8 . Okita, K. et al. (2007) Nature 448, 313-7. 9 . Arnold, K. et al. (2011) Cell Stem Cell 9, 317-29. |
Western blot analysis of extracts from NCCIT and NTERA2 cells using Sox2 Antibody.使用Sox2抗体对NCCIT和NTERA2细胞提取物进行western blot分析。 | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 NCCIT cells and either 20 μl of Sox2 Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human Oct-4 Promoter Primers #4641, SimpleChIP® Human Sox2 Promoter Primers #4649, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.使用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003将4 x 106 NCCIT细胞与20μl Sox2抗体或2μl正常兔IgG#2729进行染色质免疫共沉淀。富集到的DNA使用SimpleChIP®人Oct-4启动子引物#4641，SimpleChIP®人Sox2启动子引物#4649和SimpleChIP®人α卫星重复序列引物#4486进行荧光实时PCR定量。各样品沉淀得到的DNA量通过与input染色质总量对比获得。 | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 mouse embryonic stem cells and either 20 μl of Sox2 Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Mouse Oct-4 Promoter Primers #4653, SimpleChIP® Mouse XIST Intron 1 Primers #4659, and the negative control SimpleChIP® Mouse RPL30 Intron 2 Primers #7015. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.使用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003将4 x 106小鼠胚胎干细胞与20μl Sox2抗体或2μl正常兔IgG#2729进行染色质免疫共沉淀。富集到的DNA使用SimpleChIP®鼠Oct-4启动子引物#4653，SimpleChIP®小鼠XIST内含子1引物#4659，和阴性对照SimpleChIP®小鼠RPL30内含子2引物#7015进行荧光实时PCR定量。各样品沉淀得到的DNA量通过与input染色质总量对比获得。 | |
Western blot analysis of extracts from NCCIT, mouse embryonic stem cells (mESCs), and F9 cells using Sox2 Antibody.使用Sox2对NCCIT，小鼠胚胎干细胞（mESCs）和F9细胞提取物进行western blot分析。 |
